Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 4686178..4686881 Replicon chromosome
Accession NZ_CP060049
Organism Klebsiella pneumoniae strain NICU_2_P7

Toxin (Protein)


Gene name ykfI Uniprot ID A0A2Z3WYB7
Locus tag E4W47_RS22945 Protein ID WP_031593213.1
Coordinates 4686178..4686519 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A2Z3WZC0
Locus tag E4W47_RS22950 Protein ID WP_032410777.1
Coordinates 4686540..4686881 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4W47_RS22915 4681222..4681488 + 267 WP_004178415.1 hypothetical protein -
E4W47_RS22920 4681488..4682160 + 673 Protein_4492 DUF4400 domain-containing protein -
E4W47_RS22925 4682171..4683055 + 885 WP_004192285.1 RES domain-containing protein -
E4W47_RS22930 4683254..4683442 - 189 Protein_4494 transposase -
E4W47_RS22935 4683459..4684570 - 1112 Protein_4495 IS3 family transposase -
E4W47_RS22940 4684938..4685945 - 1008 WP_049017154.1 restriction endonuclease -
E4W47_RS22945 4686178..4686519 - 342 WP_031593213.1 TA system toxin CbtA family protein Toxin
E4W47_RS22950 4686540..4686881 - 342 WP_032410777.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
E4W47_RS22955 4686892..4687434 - 543 WP_100116935.1 DNA repair protein RadC -
E4W47_RS22960 4687447..4687887 - 441 WP_001616563.1 antirestriction protein -
E4W47_RS22965 4687918..4688739 - 822 WP_094986557.1 DUF945 domain-containing protein -
E4W47_RS22970 4688860..4689333 - 474 WP_031593204.1 hypothetical protein -
E4W47_RS22975 4689405..4689857 - 453 WP_031593203.1 hypothetical protein -
E4W47_RS22980 4689893..4690609 - 717 WP_094986556.1 WYL domain-containing protein -
E4W47_RS22985 4690853..4691728 - 876 WP_094986555.1 GTPase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4675333..4718180 42847


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-109)

Antitoxin

Antitoxin CbeA

(13-111)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12736.66 Da        Isoelectric Point: 8.0324

>T169771 WP_031593213.1 NZ_CP060049:c4686519-4686178 [Klebsiella pneumoniae]
MKTLPATTPQAATLCLSPVSVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
DWQEQSPYLRAVDILRARQATGLLKRNRISAAR

Download         Length: 342 bp

>T169771 NZ_CP076693:55984-56268 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12765.40 Da        Isoelectric Point: 7.0269

>AT169771 WP_032410777.1 NZ_CP060049:c4686881-4686540 [Klebsiella pneumoniae]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFSEAECLKLDVAFPHFISQMESMLTTGEMNPRH
AHRVTLYHNGFTCEADTLGSCGYVYIAVYSTHR

Download         Length: 342 bp

>AT169771 NZ_CP076693:55748-55981 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z3WYB7

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3WZC0

References