Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4047319..4047938 Replicon chromosome
Accession NZ_CP060049
Organism Klebsiella pneumoniae strain NICU_2_P7

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag E4W47_RS19870 Protein ID WP_002892050.1
Coordinates 4047720..4047938 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag E4W47_RS19865 Protein ID WP_002892066.1
Coordinates 4047319..4047693 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4W47_RS19855 4042471..4043664 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
E4W47_RS19860 4043687..4046833 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
E4W47_RS19865 4047319..4047693 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
E4W47_RS19870 4047720..4047938 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
E4W47_RS19875 4048101..4048667 + 567 WP_002892030.1 maltose O-acetyltransferase -
E4W47_RS19880 4048639..4048779 - 141 WP_004147370.1 hypothetical protein -
E4W47_RS19885 4048800..4049270 + 471 WP_002892026.1 YlaC family protein -
E4W47_RS19890 4049245..4050696 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
E4W47_RS19895 4050797..4051495 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
E4W47_RS19900 4051492..4051632 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
E4W47_RS19905 4051632..4051895 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T169769 WP_002892050.1 NZ_CP060049:4047720-4047938 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T169769 NZ_CP076689:4968334-4968669 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACTACGTCATATCTGTCGCCCGTCGTGGTCTGGCAAATATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCGATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTACGGGCGCGGCAGGCTACAGGTCTGCTGCGCCGCCG
CCATAACACGCCATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT169769 WP_002892066.1 NZ_CP060049:4047319-4047693 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT169769 NZ_CP076689:4967968-4968303 [Escherichia coli]
ATGCTGCAAAAGGATAAGGTCATGCAACAAGAATGTGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGCTGATGAAACAACTGGAGCTGAAACTGGCTGACGATGAACTCTCCGCCTGCCAACAACGT
ACCGTCACACTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References