Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2731639..2733227 Replicon chromosome
Accession NZ_CP060049
Organism Klebsiella pneumoniae strain NICU_2_P7

Toxin (Protein)


Gene name hipA Uniprot ID A0A486KW17
Locus tag E4W47_RS13520 Protein ID WP_004190166.1
Coordinates 2731639..2732961 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag E4W47_RS13525 Protein ID WP_002904790.1
Coordinates 2732961..2733227 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4W47_RS13505 2726695..2729196 + 2502 WP_004190161.1 fimbrial biogenesis outer membrane usher protein -
E4W47_RS13510 2729184..2730188 + 1005 WP_094986403.1 type 1 fimbrial protein -
E4W47_RS13515 2730330..2731622 + 1293 WP_002904794.1 glycoside hydrolase family 10 protein -
E4W47_RS13520 2731639..2732961 - 1323 WP_004190166.1 type II toxin-antitoxin system HipA family toxin Toxin
E4W47_RS13525 2732961..2733227 - 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
E4W47_RS13530 2733492..2733818 - 327 WP_004143476.1 hydroxyisourate hydrolase -
E4W47_RS13535 2733815..2734315 - 501 WP_004190169.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
E4W47_RS13540 2734293..2735672 - 1380 WP_004184021.1 purine permease -
E4W47_RS13545 2736018..2737172 + 1155 WP_002904783.1 FAD-dependent urate hydroxylase HpxO -
E4W47_RS13550 2737153..2738076 - 924 WP_004190172.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49171.46 Da        Isoelectric Point: 7.5357

>T169767 WP_004190166.1 NZ_CP060049:c2732961-2731639 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTTLLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T169767 NZ_CP076689:4737038-4737211 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTTTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT169767 WP_002904790.1 NZ_CP060049:c2733227-2732961 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT169767 NZ_CP076689:4736714-4737010 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486KW17

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References