Detailed information of TA system TA169751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 283368..284134
• Replicon: chromosome
• Accession: NZ_CP060047
• Organism: Vibrio vulnificus strain 2497-87

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: H6S61_RS01635
• Protein ID: WP_185891283.1
• Coordinates: 283637..284134 (+)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A347UQX1
• Locus tag: H6S61_RS01630
• Protein ID: WP_017420534.1
• Coordinates: 283368..283640 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H6S61_RS01590	278433..278531	-	99	WP_185891272.1	DUF3265 domain-containing protein	-
H6S61_RS01595	278528..279736	-	1209	WP_185890792.1	IS4 family transposase	-
H6S61_RS01600	279905..280324	+	420	WP_185891274.1	BLUF domain-containing protein	-
H6S61_RS01605	280418..280912	-	495	WP_185891277.1	GNAT family N-acetyltransferase	-
H6S61_RS01610	280934..281056	-	123	Protein_322	DUF3265 domain-containing protein	-
H6S61_RS01615	281516..282016	-	501	WP_185891279.1	GNAT family N-acetyltransferase	-
H6S61_RS01620	282635..283138	-	504	WP_185891281.1	hypothetical protein	-
H6S61_RS23030	283166..283258	-	93	WP_079741491.1	DUF3265 domain-containing protein	-
H6S61_RS01630	283368..283640	+	273	WP_017420534.1	DUF1778 domain-containing protein	Antitoxin
H6S61_RS01635	283637..284134	+	498	WP_185891283.1	GNAT family N-acetyltransferase	Toxin
H6S61_RS01640	284157..284312	-	156	WP_185891285.1	DUF3265 domain-containing protein	-
H6S61_RS01645	284385..285593	+	1209	WP_039563122.1	IS4 family transposase	-
H6S61_RS01650	285955..286101	-	147	WP_185892172.1	DUF3265 domain-containing protein	-
H6S61_RS01655	286076..286321	-	246	WP_185892174.1	Rho-binding antiterminator	-
H6S61_RS01660	286543..286764	-	222	WP_185891288.1	hypothetical protein	-
H6S61_RS01665	287151..288359	-	1209	WP_185890792.1	IS4 family transposase	-
H6S61_RS01670	288399..289064	-	666	WP_185891290.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	217740..379394	161654
	-	inside	IScluster/Tn	-	-	278528..288359	9831
	-	inside	Integron	-	-	221640..379394	157754

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-86)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18528.21 Da
• Isoelectric Point: 8.8084

>T169751 WP_185891283.1 NZ_CP060047:283637-284134 [Vibrio vulnificus]
MMNTVLLDKAKHDRNRFNCGIEALNNYLKVMASQQAKKDNTRTFVLEDDRDNSHVIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFEDAENKLFITIADV
RASLG

Nucleotide

• Length: 498 bp

>T169751 NZ_CP076689:c545058-544840 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9735.22 Da
• Isoelectric Point: 8.5960

>AT169751 WP_017420534.1 NZ_CP060047:283368-283640 [Vibrio vulnificus]
MATTLPRITARVDVDTQDLLTKAAAIAGMSSINSFVLSAAIEKAKQVIEREQALKLSQADAMLLMEALDRPATQNSKLKA
AADRYESKTQ

Nucleotide

• Length: 273 bp

>AT169751 NZ_CP076689:c545458-545084 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A347UQX1