Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relB-yefM/YoeB-RelB
Location 223147..223663 Replicon chromosome
Accession NZ_CP060047
Organism Vibrio vulnificus strain 2497-87

Toxin (Protein)


Gene name relB Uniprot ID U2ZZV8
Locus tag H6S61_RS01010 Protein ID WP_005464459.1
Coordinates 223147..223416 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A7W4CB60
Locus tag H6S61_RS01015 Protein ID WP_005483180.1
Coordinates 223409..223663 (-) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H6S61_RS00985 219055..220317 + 1263 Protein_196 hypothetical protein -
H6S61_RS00990 220328..221536 - 1209 WP_185890792.1 IS4 family transposase -
H6S61_RS00995 221643..222056 - 414 Protein_198 hypothetical protein -
H6S61_RS01000 222083..222205 - 123 WP_079764956.1 DUF3265 domain-containing protein -
H6S61_RS01005 222555..222713 - 159 WP_185892120.1 DUF3265 domain-containing protein -
H6S61_RS01010 223147..223416 - 270 WP_005464459.1 Txe/YoeB family addiction module toxin Toxin
H6S61_RS01015 223409..223663 - 255 WP_005483180.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
H6S61_RS01020 224211..224402 - 192 WP_185892123.1 DUF3265 domain-containing protein -
H6S61_RS01025 224706..224834 - 129 WP_185892125.1 DUF3265 domain-containing protein -
H6S61_RS01030 225296..225913 - 618 WP_185891212.1 hypothetical protein -
H6S61_RS01035 226118..226420 - 303 WP_038969961.1 hypothetical protein -
H6S61_RS01040 226440..226562 - 123 WP_185892128.1 DUF3265 domain-containing protein -
H6S61_RS01045 226559..227002 - 444 WP_017420781.1 GNAT family N-acetyltransferase -
H6S61_RS01050 227175..227465 - 291 WP_000509889.1 hypothetical protein -
H6S61_RS01055 227485..227607 - 123 WP_185891214.1 DUF3265 domain-containing protein -
H6S61_RS01060 227636..228022 - 387 WP_185891216.1 tautomerase family protein -
H6S61_RS01065 228001..228201 - 201 WP_185891218.1 DUF3265 domain-containing protein -
H6S61_RS01070 228174..228503 - 330 WP_185891220.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 217740..379394 161654
- inside Integron - - 221640..379394 157754


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(10-89)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10614.96 Da        Isoelectric Point: 7.9816

>T169746 WP_005464459.1 NZ_CP060047:c223416-223147 [Vibrio vulnificus]
MSSSQRLLSWTDDAWDDYLYWQTQDKKTLKRINKLINDVKRSPFEGIGKPEPLKENLSGFWSRRIDDTNRLVYAVDDQAI
TIISCRYHY

Download         Length: 270 bp

>T169746 NZ_CP076687:c4762971-4762594 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9481.66 Da        Isoelectric Point: 5.0623

>AT169746 WP_005483180.1 NZ_CP060047:c223663-223409 [Vibrio vulnificus]
MRIVSFTEARNGLKAVLDGVVNDADTTVITRRDSEDAVVMSLDYYNSLMETVHLLRSPQNVEHLNRSIAQYRAGKTTARE
LIDE

Download         Length: 255 bp

>AT169746 NZ_CP076687:c4763429-4763061 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCATGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCAAT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U2ZZV8

Antitoxin

Source ID Structure
AlphaFold DB A0A7W4CB60

References