Detailed information of TA system TA169744

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 46625..47274
• Replicon: plasmid pBCPS27
• Accession: NZ_CP060042
• Organism: Burkholderia cenocepacia strain PS27

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SY91_RS34330
• Protein ID: WP_011695140.1
• Coordinates: 46625..47035 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A4S3GW89
• Locus tag: SY91_RS34335
• Protein ID: WP_011695139.1
• Coordinates: 47032..47274 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SY91_RS34280	41663..41908	-	246	WP_011695149.1	hypothetical protein	-
SY91_RS34285	41905..42288	-	384	WP_041489702.1	hypothetical protein	-
SY91_RS34290	42300..43385	-	1086	WP_011695147.1	hypothetical protein	-
SY91_RS34295	43413..43754	-	342	WP_041489701.1	hypothetical protein	-
SY91_RS34300	44068..44550	-	483	WP_011695145.1	hypothetical protein	-
SY91_RS34305	44574..44780	-	207	WP_011695144.1	hypothetical protein	-
SY91_RS34310	44807..45529	-	723	WP_011695143.1	helix-turn-helix transcriptional regulator	-
SY91_RS34315	45715..45915	-	201	WP_185921524.1	hypothetical protein	-
SY91_RS34320	45964..46311	-	348	WP_185921535.1	hypothetical protein	-
SY91_RS34325	46403..46543	-	141	WP_185921525.1	hypothetical protein	-
SY91_RS34330	46625..47035	-	411	WP_011695140.1	type II toxin-antitoxin system VapC family toxin	Toxin
SY91_RS34335	47032..47274	-	243	WP_011695139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SY91_RS34340	47396..48426	-	1031	Protein_50	DNA ligase	-
SY91_RS34345	48883..49554	+	672	WP_011695137.1	AAA family ATPase	-
SY91_RS34350	49554..50519	+	966	WP_185921526.1	ParB/RepB/Spo0J family partition protein	-
SY91_RS34355	51245..52102	+	858	WP_006482109.1	replication initiator protein A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..160144	160144

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• SpoVT-AbrB domain (12-50)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14773.00 Da
• Isoelectric Point: 5.1526

>T169744 WP_011695140.1 NZ_CP060042:c47035-46625 [Burkholderia cenocepacia]
MTLYLLDTNILSNVIRDPRGACATRIGETPPEQVCTSIVVAAELRFGVWKRGSSTLAQRVEQLLASLTVLPLQPDADRCY
GRLRAELEKQGQLIGANDMLIAAHALAVDAVLVTDNTAEFTRVAGLPVENWLRSAT

Nucleotide

• Length: 411 bp

>T169744 NZ_CP076687:4401678-4401989 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9290.53 Da
• Isoelectric Point: 4.6856

>AT169744 WP_011695139.1 NZ_CP060042:c47274-47032 [Burkholderia cenocepacia]
MPTHDARHARLFRNGRSQAVRIPREFELPVSEVTIHREGRRLIIEPVEAAPLRELFAQWAPLDETFPDIEDLPPEPVDIE

Nucleotide

• Length: 243 bp

>AT169744 NZ_CP076687:4401990-4402280 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A4S3GW89