Detailed information of TA system TA169741

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1905547..1906112
• Replicon: chromosome
• Accession: NZ_CP060040
• Organism: Burkholderia cenocepacia strain PS27

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SY91_RS24915
• Protein ID: WP_023477059.1
• Coordinates: 1905741..1906112 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7G6V745
• Locus tag: SY91_RS24910
• Protein ID: WP_023477058.1
• Coordinates: 1905547..1905744 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SY91_RS24885	1900884..1901339	-	456	WP_006480483.1	RbsD/FucU family protein	-
SY91_RS24890	1901418..1901810	-	393	WP_023477055.1	nuclear transport factor 2 family protein	-
SY91_RS24895	1901993..1903546	-	1554	WP_023477056.1	galactarate dehydratase	-
SY91_RS24900	1903776..1904690	+	915	WP_006480481.1	5-dehydro-4-deoxyglucarate dehydratase	-
SY91_RS24905	1904807..1905361	-	555	WP_023477057.1	hypothetical protein	-
SY91_RS24910	1905547..1905744	+	198	WP_023477058.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
SY91_RS24915	1905741..1906112	+	372	WP_023477059.1	PIN domain-containing protein	Toxin
SY91_RS24920	1906240..1907817	-	1578	WP_023477060.1	aldehyde dehydrogenase (NADP(+))	-
SY91_RS24925	1908104..1909456	+	1353	WP_175769605.1	MFS transporter	-
SY91_RS24930	1909522..1910247	-	726	WP_006480476.1	site-2 protease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-46)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13517.59 Da
• Isoelectric Point: 5.8921

>T169741 WP_023477059.1 NZ_CP060040:1905741-1906112 [Burkholderia cenocepacia]
MILVDTSVWIDHLRAGDATLTALLEAERVLIHPFIVGELALGSMHNRETVLDALRDLPAATLATDDEVQRMIDAVPLHGL
GIGYVDAHLLASARLTSDSRLWTRDRRLLAAAERLQLVARPAH

Nucleotide

• Length: 372 bp

>T169741 NZ_CP076687:c3904130-3903603 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7070.14 Da
• Isoelectric Point: 10.6826

>AT169741 WP_023477058.1 NZ_CP060040:1905547-1905744 [Burkholderia cenocepacia]
MRTTLSLDDALLAKAQQLTGVTEKSALVREALRALIARESARRLARLGGTEPDLESVPRRPSELA

Nucleotide

• Length: 198 bp

>AT169741 NZ_CP076687:c3904403-3904137 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7G6V745