Detailed information of TA system TA169740

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 1514328..1515554
• Replicon: chromosome
• Accession: NZ_CP060040
• Organism: Burkholderia cenocepacia strain PS27

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SY91_RS23275
• Protein ID: WP_185921171.1
• Coordinates: 1514973..1515554 (+)
• Length: 194 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: SY91_RS23270
• Protein ID: WP_006480773.1
• Coordinates: 1514328..1514894 (+)
• Length: 189 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SY91_RS23245	1509467..1509949	-	483	WP_006480777.1	cupin domain-containing protein	-
SY91_RS23250	1510260..1510913	+	654	WP_105797880.1	cysteine hydrolase	-
SY91_RS23255	1510939..1511670	-	732	WP_006498851.1	ribonuclease T2	-
SY91_RS23260	1511798..1512376	-	579	WP_185921169.1	hypothetical protein	-
SY91_RS23265	1512476..1513879	-	1404	WP_185921170.1	DUF308 domain-containing protein	-
SY91_RS23270	1514328..1514894	+	567	WP_006480773.1	helix-turn-helix domain-containing protein	Antitoxin
SY91_RS23275	1514973..1515554	+	582	WP_185921171.1	HD domain-containing protein	Toxin
SY91_RS23280	1515964..1517157	+	1194	WP_185921172.1	efflux RND transporter periplasmic adaptor subunit	-
SY91_RS23285	1517160..1519214	+	2055	WP_006480770.1	MacB family efflux pump subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (6-153)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-64)

Sequences

Toxin

Protein

• Length: 194 a.a.
• Molecular weight: 21366.03 Da
• Isoelectric Point: 5.3083

>T169740 WP_185921171.1 NZ_CP060040:1514973-1515554 [Burkholderia cenocepacia]
MNPDTIHARLAFLREAERLKDVLRSGHTSAGRPESTAEHSWRLCLMALVFADALPGVDTLKLLKLCVVHDLGEALHGDIP
AIEQAAHPDKSAHERDDLLTLTASLDRAVRDEIVALWDEYEAAASPEARAAKALDKLETILQHNQGSNPPDFDYAFNLGY
GRRYTDAAPLFRAIREIVDADTQRKIDTGGYRA

Nucleotide

• Length: 582 bp

>T169740 NZ_CP076687:3592682-3593146 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 189 a.a.
• Molecular weight: 20485.35 Da
• Isoelectric Point: 10.7076

>AT169740 WP_006480773.1 NZ_CP060040:1514328-1514894 [Burkholderia cenocepacia]
MPHPPLHPSSLGPQLKRWRTLHRVKQSHAAELFGVAQSTISRWEAGLQQMSPAERATAERLLAARLDSAGDHALARLIAG
SAGRMHLVCDLTHRLLASSPARAAEFSQPLSTLLGTSLWRYATPEIARMEAALDPLGWHDRAGPPSVEFDTGANASRVVP
IRGSRCRWTRLTLSDGSAVRLVETLDAH

Nucleotide

• Length: 567 bp

>AT169740 NZ_CP076687:3592347-3592682 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA