Detailed information of TA system TA169737

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1252716..1254481
• Replicon: chromosome
• Accession: NZ_CP060040
• Organism: Burkholderia cenocepacia strain PS27

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: SY91_RS22075
• Protein ID: WP_023475912.1
• Coordinates: 1253570..1254481 (-)
• Length: 304 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A7G6V5I1
• Locus tag: SY91_RS22070
• Protein ID: WP_006478727.1
• Coordinates: 1252716..1253573 (-)
• Length: 286 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SY91_RS22050	1247879..1249369	-	1491	WP_023475910.1	aldehyde dehydrogenase	-
SY91_RS22055	1249442..1250269	-	828	WP_006478730.1	aspartate dehydrogenase	-
SY91_RS22060	1250262..1251929	-	1668	WP_006478729.1	thiamine pyrophosphate-binding protein	-
SY91_RS22065	1251922..1252719	-	798	WP_023475911.1	SDR family oxidoreductase	-
SY91_RS22070	1252716..1253573	-	858	WP_006478727.1	IclR family transcriptional regulator	Antitoxin
SY91_RS22075	1253570..1254481	-	912	WP_023475912.1	alpha/beta fold hydrolase	Toxin
SY91_RS22080	1254500..1255027	-	528	WP_006489904.1	cupin domain-containing protein	-
SY91_RS22085	1255062..1255832	-	771	WP_023475913.1	SDR family oxidoreductase	-
SY91_RS22090	1255825..1256895	-	1071	WP_006478723.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
SY91_RS22095	1256956..1257267	-	312	WP_006478722.1	hypothetical protein	-
SY91_RS22100	1257269..1257586	-	318	WP_006478721.1	non-heme iron oxygenase ferredoxin subunit	-
SY91_RS22105	1258029..1259108	+	1080	WP_012339772.1	porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (56-284)

Antitoxin

• Transcription regulator IclR, C-terminal (143-266)

Sequences

Toxin

Protein

• Length: 304 a.a.
• Molecular weight: 30997.31 Da
• Isoelectric Point: 7.7324

>T169737 WP_023475912.1 NZ_CP060040:c1254481-1253570 [Burkholderia cenocepacia]
MSVIDKRERDRTAAFAALLGQFPEQRCAAGAAGTIGYREAGAGEAGAQHAGRALPVVLLHGIGSGAASWVRQLDALGASR
RVLAWDAPGYGVSTPVHGASPAAADYAASLNAWLEALGIERCVLVGHSLGAIIAGGLVRVMPARIAGLLLISPAGGYGSA
PADTRESRRDARLAMLAELGPAGLAEQRSGNMLSGFANEEAKAWVRWNMARIVPAGYAQATHLLANADLAADLAGFRGRT
AVAVGANDAITPPAACERIAAAAHVGLQVIPQAGHAGYVEAPAVYSALIDTFCRQCDPQRGLA

Nucleotide

• Length: 912 bp

>T169737 NZ_CP076687:c3232414-3232007 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 286 a.a.
• Molecular weight: 31366.81 Da
• Isoelectric Point: 6.6159

>AT169737 WP_006478727.1 NZ_CP060040:c1253573-1252716 [Burkholderia cenocepacia]
MSEPLQQNESDHAKAEASYVVPGLERGLRILAEFSPREPVLGAPELSKRLGIPRTTVFRLLQTLESLGFLERADKDRNYK
LGIAVLRLGFEYLSSLELTDLGLPVIESLRDATGFTTHIVIRDGRDVVFVAKAQSQSPVFSSIRVNVGTRLPAHATTHGH
VLMGDLSLKELHALYPEGALNRMTSATPETVDALYDVIREDALRGYGVSNSSFERGISVVTAPVRNETQRIVACITVTVP
RPEIDAALIADGLIDKVQRAAAELSRRLNYRSDDEHTFLKALGLR

Nucleotide

• Length: 858 bp

>AT169737 NZ_CP076687:c3232661-3232395 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTTTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7G6V5I1