Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 854464..855097 Replicon chromosome
Accession NZ_CP060040
Organism Burkholderia cenocepacia strain PS27

Toxin (Protein)


Gene name vapC Uniprot ID A0A3N9EG56
Locus tag SY91_RS20335 Protein ID WP_023477876.1
Coordinates 854684..855097 (+) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A807EDB2
Locus tag SY91_RS20330 Protein ID WP_027808267.1
Coordinates 854464..854697 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SY91_RS20315 849523..851216 + 1694 Protein_744 VRR-NUC domain-containing protein -
SY91_RS20320 851213..853513 + 2301 WP_023477877.1 ATP-dependent DNA helicase -
SY91_RS20325 853529..854302 - 774 WP_006485222.1 helix-turn-helix domain-containing protein -
SY91_RS20330 854464..854697 + 234 WP_027808267.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
SY91_RS20335 854684..855097 + 414 WP_023477876.1 PIN domain-containing protein Toxin
SY91_RS20340 855170..856471 - 1302 WP_023477875.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
SY91_RS20345 856676..857983 - 1308 WP_011547704.1 shikimate transporter -
SY91_RS20350 858341..859468 + 1128 WP_023477874.1 VOC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(10-119)

Antitoxin

SpoVT-AbrB domain

(5-37)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 15637.91 Da        Isoelectric Point: 5.6324

>T169733 WP_023477876.1 NZ_CP060040:854684-855097 [Burkholderia cenocepacia]
MSRIETEKSFVDSNVVLYLLSEDETKANRAESLFLRRPTISVQVLNEVASVCNRKLRMPWREVGKFLEPIRSLCHVVPLT
VDAHDLARRLAEWHRLSFYDACIVATASIEGCEYLYTEDMHDGLQVDGGPVLRNPFA

Download         Length: 414 bp

>T169733 NZ_CP076687:c1940556-1940454 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8807.99 Da        Isoelectric Point: 5.1468

>AT169733 WP_027808267.1 NZ_CP060040:854464-854697 [Burkholderia cenocepacia]
MQVAKWGNSLAVRLPASVVDALELREGDDIEIVVDSPRVFAVSRKPRPDELLERLRRFRGKLPADFKFSRDDANEQD

Download         Length: 234 bp

>AT169733 NZ_CP076687:1940416-1940684 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3N9EG56

Antitoxin

Source ID Structure
AlphaFold DB A0A807EDB2

References