Detailed information of TA system TA169729

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 83974..85478
• Replicon: plasmid p1
• Accession: NZ_CP060037
• Organism: Sphingobium barthaii strain KK22

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: A0A7M2GQ81
• Locus tag: H5V43_RS21770
• Protein ID: WP_025547151.1
• Coordinates: 84204..85478 (+)
• Length: 425 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: H5V43_RS21765
• Protein ID: WP_025547149.1
• Coordinates: 83974..84207 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5V43_RS21750	82030..82269	+	240	Protein_74	hypothetical protein	-
H5V43_RS21755	82499..83407	+	909	WP_128830679.1	acetyl-CoA C-acyltransferase	-
H5V43_RS21760	83499..83803	+	305	Protein_76	alkylphosphonate utilization protein	-
H5V43_RS21765	83974..84207	+	234	WP_025547149.1	helix-turn-helix transcriptional regulator	Antitoxin
H5V43_RS21770	84204..85478	+	1275	WP_025547151.1	type II toxin-antitoxin system HipA family toxin	Toxin
H5V43_RS21775	85360..86469	-	1110	WP_150295611.1	tyrosine-type recombinase/integrase	-
H5V43_RS21780	86703..87050	+	348	WP_037484504.1	transposase	-
H5V43_RS21785	86999..87847	+	849	WP_146032627.1	IS3 family transposase	-
H5V43_RS21790	87888..89573	+	1686	WP_128830731.1	DDE-type integrase/transposase/recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..387592	387592

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (9-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-63)

Sequences

Toxin

Protein

• Length: 425 a.a.
• Molecular weight: 45093.89 Da
• Isoelectric Point: 7.8188

>T169729 WP_025547151.1 NZ_CP060037:84204-85478 [Sphingobium barthaii]
VSTDILDRLIVWSDQHIAGELSIDRAGAMRFSYAPDWLADPDRTALSQAMPKRVESFGDALCKAVFGGLLPEEGQRTAIA
RALGVSPDNPFRLLAALGGDVAGALAFLPEGEMPPAAPAGRPARPLDDTELAALIARLPRVPMLAGEGGARLSLAGAQSK
LPVVLVKGAIALPRVGEPSTHLIKSEPDRFPGLAANEAFCLALARSVGLDAVMAEWRAVAGKGYLLVTRYDRTVQPGATS
RLHQEDFAQALGVPSNRKYASEGGPTFRDGFALVRAATSRPAREVLKLADAAIFNVIIGNADAHAKNYSLLRRANGEVVL
APLYDLVATHVWPELSPKLAMRFGRAATLEDLDAASFVRFAQDAGLALPFLRRRAAAIAARVQAAITDRVEIPGLEDTAC
LEDLPATVLDRAMRLSLKAEARGR

Nucleotide

• Length: 1275 bp

>T169729 NZ_CP076687:c508231-508013 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8292.80 Da
• Isoelectric Point: 7.9955

>AT169729 WP_025547149.1 NZ_CP060037:83974-84207 [Sphingobium barthaii]
MLDMITIGRIVRDERKELGLRQDELAAASGVGLRFIVEVERGKPTAQIGKVIAVLAALGCELEIRRPDGIVIAGRPA

Nucleotide

• Length: 234 bp

>AT169729 NZ_CP076687:c508631-508257 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A7M2GQ81