Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 147768..148365 Replicon chromosome
Accession NZ_CP060035
Organism Sphingobium barthaii strain KK22

Toxin (Protein)


Gene name hicA Uniprot ID A0A7M2GGB0
Locus tag H5V43_RS00720 Protein ID WP_025547159.1
Coordinates 148183..148365 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicA Uniprot ID A0A2K0Y5R5
Locus tag H5V43_RS00715 Protein ID WP_025547157.1
Coordinates 147768..148160 (-) Length 131 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5V43_RS00675 143030..143758 - 729 WP_025552238.1 IS21-like element helper ATPase IstB -
H5V43_RS00680 143745..143906 - 162 Protein_132 IS21 family transposase -
H5V43_RS00685 144097..144513 + 417 WP_025552235.1 hypothetical protein -
H5V43_RS00690 144670..145226 + 557 Protein_134 hypothetical protein -
H5V43_RS00695 145223..145771 + 549 WP_025552231.1 hypothetical protein -
H5V43_RS00700 146189..147037 - 849 WP_146032627.1 IS3 family transposase -
H5V43_RS00705 146986..147333 - 348 WP_037484504.1 transposase -
H5V43_RS00710 147422..147721 + 300 WP_162195971.1 hypothetical protein -
H5V43_RS00715 147768..148160 - 393 WP_025547157.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H5V43_RS00720 148183..148365 - 183 WP_025547159.1 type II toxin-antitoxin system HicA family toxin Toxin
H5V43_RS00725 148561..148896 + 336 WP_052816652.1 HIRAN domain-containing protein -
H5V43_RS00730 148918..149634 + 717 WP_128830690.1 Crp/Fnr family transcriptional regulator -
H5V43_RS00735 149763..150214 - 452 Protein_143 DUF3363 domain-containing protein -
H5V43_RS00740 150608..151054 + 447 WP_099186196.1 Arm DNA-binding domain-containing protein -
H5V43_RS00745 151916..152548 - 633 WP_025547168.1 VOC family protein -
H5V43_RS00750 152622..153206 - 585 WP_025547170.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 143030..143758 728


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(5-122)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6896.10 Da        Isoelectric Point: 11.0046

>T169728 WP_025547159.1 NZ_CP060035:c148365-148183 [Sphingobium barthaii]
MERDSKKIVRRLLEEGYEQVSVKGSHHKFRKGDRTIIVPHPKKDLPLGTARSIAKMAGWL

Download         Length: 183 bp

>T169728 NZ_CP076687:324392-325312 [Escherichia coli]
ATGGATCTGCGTGATAGCTATGCCAGGCAGGTTAAACTGTTGATGGCCGCTCTGCCGCATGTGGCGAAGGAATCCTGTTT
CGCGTTGAAAGGCGGCACAGCAATCAATTTATTTGTGCAGGATTTCCCCCGTCTATCGGTGGATATCGATCTGGCGTATA
AGAGCTTTGCGGATCGCGATACCGATCTGGCGGCGATCAACGACGCTTTGAAGAGAATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATATGTGCTGCGCTTGATCGTCAGCAT
CCGCGCGATCTGTTTGATGTGTTGAATATGCTGGGGAGACCGGGCCTGACGCGCGAAATTTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGTCCCATTGCCGAGTTGCTGGCTTCAAACTGGGATACAGATCGGATTGCCACGTTATACGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTTCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATTTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGATGCTTAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGAGTAA

Antitoxin


Download         Length: 131 a.a.        Molecular weight: 14376.24 Da        Isoelectric Point: 4.4634

>AT169728 WP_025547157.1 NZ_CP060035:c148160-147768 [Sphingobium barthaii]
MKYFYAIVHKDADSAFGVSFPDLPGCFSAADRQEDVLPNAVEALELWFEDAEEVDPRPVDQVREAVKEDLAQGAFLLAVP
RITSANKLARVNLSLDRGMLDAIDQAAALRKLTRSAFMAEAARNEIEGRH

Download         Length: 393 bp

>AT169728 NZ_CP076687:323623-324405 [Escherichia coli]
TTGCCAAACTATCTAAACTGGTTGATGCAAAACACGCTACCCGGTCAGGTGCTGCTCCAGTCATGGCTTACCCGCAATGG
CGTAGATCGTAAGCTTTCTTATCTCTATGTTCAGAATGGCTGGCTCAGGCGCATCGCTCACGGTGTGTACTGCCGGACGG
GCCGTGAGCCGGATTGGGTAGACGCGGTTTACTGCCTTCAAACACAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGCCATGCGCACTATACCGAACCGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTGCTGCCTTCGAACAATCAGCAACATTTATCACGCTGTATACATCGGGCCTTACCGTTGATGTCT
CGTCATTTACTACGGAACTGAAAATTGGCGACCGGCAGTTAGCAGGCAGTGCACCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTGATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGGGGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGTCATTCGATTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATCTTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCTGGTGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCCCGCACATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7M2GGB0

Antitoxin

Source ID Structure
AlphaFold DB A0A2K0Y5R5

References