Detailed information of TA system TA169727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 140697..141541
• Replicon: chromosome
• Accession: NZ_CP060035
• Organism: Sphingobium barthaii strain KK22

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: H5V43_RS00660
• Protein ID: WP_193666723.1
• Coordinates: 141026..141541 (+)
• Length: 172 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A7M2GGC7
• Locus tag: H5V43_RS00655
• Protein ID: WP_025551515.1
• Coordinates: 140697..141005 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5V43_RS00650	136809..139778	+	2970	WP_025551514.1	relaxase domain-containing protein	-
H5V43_RS00655	140697..141005	+	309	WP_025551515.1	DUF1778 domain-containing protein	Antitoxin
H5V43_RS00660	141026..141541	+	516	WP_193666723.1	GNAT family N-acetyltransferase	Toxin
H5V43_RS00665	141669..142016	+	348	WP_037484504.1	transposase	-
H5V43_RS00670	141965..142813	+	849	WP_146032627.1	IS3 family transposase	-
H5V43_RS00675	143030..143758	-	729	WP_025552238.1	IS21-like element helper ATPase IstB	-
H5V43_RS00680	143745..143906	-	162	Protein_132	IS21 family transposase	-
H5V43_RS00685	144097..144513	+	417	WP_025552235.1	hypothetical protein	-
H5V43_RS00690	144670..145226	+	557	Protein_134	hypothetical protein	-
H5V43_RS00695	145223..145771	+	549	WP_025552231.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	143030..143758	728

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (47-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (21-96)

Sequences

Toxin

Protein

• Length: 172 a.a.
• Molecular weight: 18424.21 Da
• Isoelectric Point: 7.3847

>T169727 WP_193666723.1 NZ_CP060035:141026-141541 [Sphingobium barthaii]
MTTRAVAAPTPLRAEHDAAAFASGQHDTLDSWLRQRALTSEGLSARTYVICDAAAPQRIVGYYTITTAMEQRAALPSAKL
RRAMPEQVPLLLVARLAVDREWQGLGLGGDLLGDALRRCFAASVIAGARGIAVHAIDEAAARFYEHHGFLRSPLGELTLI
LPIEALRGIID

Nucleotide

• Length: 516 bp

>T169727 NZ_CP076687:55985-56269 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11729.45 Da
• Isoelectric Point: 10.7320

>AT169727 WP_025551515.1 NZ_CP060035:140697-141005 [Sphingobium barthaii]
MARAESHGRERRATARKDDVIQVRAMAGTKALLNRAAAMRGQKLSEFMLESARREAENALLDQRTFFLDDERHAAFVVML
DAPHTPSEKLKELMHRKPAWDR

Nucleotide

• Length: 309 bp

>AT169727 NZ_CP076687:55749-55982 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A7M2GGC7