Detailed information of TA system TA169724

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 16800..17356
• Replicon: plasmid pBY1
• Accession: NZ_CP060031
• Organism: Aeromonas salmonicida subsp. masoucida strain BR19001YR

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A2H9U809
• Locus tag: G7042_RS22810
• Protein ID: WP_042468953.1
• Coordinates: 17081..17356 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: G7042_RS22805
• Protein ID: WP_174416604.1
• Coordinates: 16800..17081 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G7042_RS22790	12287..12961	-	675	Protein_15	IS21 family transposase	-
G7042_RS22795	12996..16024	-	3029	Protein_16	Tn3 family transposase	-
G7042_RS22800	16028..16588	-	561	WP_042468955.1	recombinase family protein	-
G7042_RS22805	16800..17081	+	282	WP_174416604.1	ribbon-helix-helix domain-containing protein	Antitoxin
G7042_RS22810	17081..17356	+	276	WP_042468953.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G7042_RS22815	18143..19057	+	915	WP_042468951.1	TOMM precursor leader peptide-binding protein	-
G7042_RS22820	19059..19244	+	186	WP_192580970.1	hypothetical protein	-
G7042_RS22825	19293..20201	+	909	Protein_22	IS5-like element ISAs4 family transposase	-
G7042_RS22830	20403..21434	+	1032	WP_088814095.1	IS630 family transposase	-
G7042_RS22835	21409..21603	+	195	WP_088814094.1	YcaO-like family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ascQ / ascR / ascS / ascT / ascU / sycO / aopO / ati2 / ati1 / ascL / ascK / ascJ / ascI / ascH / ascG / ascF / ascE / ascD / ascC / ascB / exsD / exsA / exsB / exsE / exsC / aopD / aopB / acrH / acrV / acrG / acrR / ascV / ascY / ascX / acr2 / acr1 / aopN / ascN / ascO / ascP	1..66892	66892
	-	inside	IScluster/Tn	-	sycO / aopO / ati2 / ati1	3829..43208	39379

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-50)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10685.43 Da
• Isoelectric Point: 9.5291

>T169724 WP_042468953.1 NZ_CP060031:17081-17356 [Aeromonas salmonicida subsp. masoucida]
MELKWTSKALSDLARLYEFLALANKPAAARAVQALTQAPTILLTNPRIGEQLFQFEPREVRRILVGEYEVRYEILDSIIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T169724 NZ_CP076679:4876833-4877111 [Rhodopseudomonas palustris]
ATGATCCGCTCGTTCCGAAATCCGGCGACCGCGCGGCTGTTTGCTGATGAGGACGTGCCGCGGTTTCGCGCGATCGAACG
GCAAGCTCGTCGAAAGCTGCTGCTGCTGGACGGCGCCGCCAAACTCGACGATTTGCGGCAACCTCCCGGCAACAGGCTCG
AGGCCTTGAAGGGCGACCGCCGCGGCCAATACTCGATCCGCATCAACGATCAATGGCGGATTTGTTTCGAGTGGCGTGAG
GACGGCGCAGAAATGGTCGAAATCGTGGACTATCATTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10351.80 Da
• Isoelectric Point: 5.6624

>AT169724 WP_174416604.1 NZ_CP060031:16800-17081 [Aeromonas salmonicida subsp. masoucida]
MKGNMTQATTKVLTAHVPLPMADKVDQMAARLERSRGWIMKQALSAWLAQEEDRDRLTQEALADVDAGRVIDHLAVQAWA
DSLNTDQPLSVPR

Nucleotide

• Length: 282 bp

>AT169724 NZ_CP076679:4877123-4877428 [Rhodopseudomonas palustris]
ATGGCCAAACCAAAGCAGCTCCCCCCGATCCCTCCCGGCGAGATTCTGATCGAGGAATTCATGCGCCCGAACGGGATCAG
TCAGAACCGGCTGGCGCGCGATATCGATATCAATCCGGCCCGGGTCAACGACATCGTGCACGGCCGCTCTGCGATCACCG
CCGCCGTCGCCCTGCGGCTGGCGAAGTACTTCGGCACGACGCCCGAACTCTGGATGAACCTGCAGGCGTCCTACGACCTG
CGCCGTGCCCGCGCCGCAGACTGGCCCGCGATTGAGCCGCGCGTCCGCGTGCTCGCCGCGGAGTAG

Structures

Toxin

• AlphaFold DB: A0A2H9U809