Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 4440476..4441116 Replicon chromosome
Accession NZ_CP060030
Organism Aeromonas salmonicida subsp. masoucida strain BR19001YR

Toxin (Protein)


Gene name vagD Uniprot ID A0A807Z4U5
Locus tag G7042_RS21075 Protein ID WP_005320982.1
Coordinates 4440476..4440889 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A807Z5T0
Locus tag G7042_RS21080 Protein ID WP_005320985.1
Coordinates 4440886..4441116 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G7042_RS21050 4436848..4437429 + 582 Protein_4079 subclass B2 metallo-beta-lactamase -
G7042_RS21055 4437616..4438533 + 918 WP_088814123.1 IS5-like element ISAs4 family transposase -
G7042_RS21060 4438768..4439685 + 918 WP_076611341.1 IS5-like element ISAs4 family transposase -
G7042_RS21065 4439743..4440018 - 276 Protein_4082 hypothetical protein -
G7042_RS21070 4440045..4440398 - 354 WP_017413138.1 hypothetical protein -
G7042_RS21075 4440476..4440889 - 414 WP_005320982.1 PIN domain-containing protein Toxin
G7042_RS21080 4440886..4441116 - 231 WP_005320985.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
G7042_RS21085 4441185..4441759 - 575 Protein_4086 site-specific integrase -
G7042_RS21090 4442290..4443207 - 918 WP_076611341.1 IS5-like element ISAs4 family transposase -
G7042_RS21095 4443275..4443676 + 402 Protein_4088 IS4 family transposase -
G7042_RS21100 4443749..4445140 - 1392 WP_034283822.1 T3SS effector protein-tyrosine-phosphatase AopH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - aopH / aerA/act 4437616..4453650 16034
- inside IScluster/Tn - aopH 4435380..4453088 17708


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-42)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 14935.23 Da        Isoelectric Point: 5.7146

>T169723 WP_005320982.1 NZ_CP060030:c4440889-4440476 [Aeromonas salmonicida subsp. masoucida]
VKTFMLDTCICSFIMREMPQSVLERLGAEVASGNRIVISAITYAEMRYGQIGKKASPKLGPAIDEFVRRLDGILPWDAAT
VDQTLEVRQQLAALGTPIGNNDAAIAAHALTAGCVLVTNNTREFSRVAGLLYEDWVH

Download         Length: 414 bp

>T169723 NZ_CP076679:c4384992-4384591 [Rhodopseudomonas palustris]
GTGACGCGCTATATGCTCGATACCAACATTCTCTCCGATCTGATCCGCAATCCGCACGGCGCTGCTGTGCGCCGCATCGC
CGAGGTCGGGGAGGGCAACGTCTGCACGAGTATCATCGTCGCCGCCGAACTCCGCTACGGCTGTGCCAAGAGCGGGTCAG
TCGAATTGACCAAGGCCGTGGCCGATCTGCTCGAGGAAATCGACGTGCTGCCGTTCGAGCCGCCCGCCGACGAAGCCTAT
GGGGCGATCCGCTCATCGCTTGAAGCAGCAGGCACGCCGATCGGCAGCAACGACCTCTTGATCGCAGCTCATGCCAAGTC
GCTCGGTGCGACCATCGTCACCGCGAACACGCAGGAGTTTGGTCGCGTGCAGGGTTTGGCAGTCGAGAACTGGCTGGCGT
GA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8699.88 Da        Isoelectric Point: 4.7419

>AT169723 WP_005320985.1 NZ_CP060030:c4441116-4440886 [Aeromonas salmonicida subsp. masoucida]
MRTVSIFKNGKNQAVRLPKDMEFQGVNDLEIIKNGDTITLRPARPDWFSLAECQKVDADFLQERTDVISDEGRFAL

Download         Length: 231 bp

>AT169723 NZ_CP076679:c4385225-4384989 [Rhodopseudomonas palustris]
ATGACCGATCGGCGGCACGTCCGTCTGTTCCGTAACGGCCGCAATCAGGCCGTTCGCATTCCGGTGGAATTCGAGATGCC
CGGCAACGAGGCGATCATGCACCGCGAGGGGGATCGGCTGGTGATCGAGCCGGTCCGCAAACGCGGACTGATCGCTTTGT
TGCAATCGATGACGCCGGTCGACGAGCCGTTCCCCGCCATCGACGATCCCGCGCCGGCCCCGGAGAAGGTGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A807Z4U5

Antitoxin

Source ID Structure
AlphaFold DB A0A807Z5T0

References