Detailed information of TA system TA169714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 3118576..3119581
• Replicon: chromosome
• Accession: NZ_CP060016
• Organism: Mycolicibacterium boenickei strain DSM 44677

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: H5U98_RS15060
• Protein ID: WP_077740740.1
• Coordinates: 3118576..3119154 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: H5U98_RS15065
• Protein ID: WP_077740739.1
• Coordinates: 3119192..3119581 (-)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5U98_RS15030 (H5U98_14980)	3114667..3115083	-	417	WP_077740745.1	hypothetical protein	-
H5U98_RS15035 (H5U98_14985)	3115177..3115692	+	516	WP_077740744.1	hypothetical protein	-
H5U98_RS15040 (H5U98_14990)	3115798..3116592	+	795	WP_097926360.1	thioesterase family protein	-
H5U98_RS15045 (H5U98_14995)	3116589..3117194	-	606	WP_077740742.1	class I SAM-dependent methyltransferase	-
H5U98_RS15050 (H5U98_15000)	3117244..3117882	-	639	WP_174814501.1	FKBP-type peptidyl-prolyl cis-trans isomerase	-
H5U98_RS15055 (H5U98_15005)	3117922..3118557	+	636	WP_077740741.1	histidine phosphatase family protein	-
H5U98_RS15060 (H5U98_15010)	3118576..3119154	-	579	WP_077740740.1	zeta toxin family protein	Toxin
H5U98_RS15065 (H5U98_15015)	3119192..3119581	-	390	WP_077740739.1	hypothetical protein	Antitoxin
H5U98_RS15070 (H5U98_15020)	3119651..3120667	-	1017	WP_077740738.1	TIGR03617 family F420-dependent LLM class oxidoreductase	-
H5U98_RS15075 (H5U98_15025)	3120818..3122173	+	1356	WP_077740737.1	alpha/beta hydrolase-fold protein	-
H5U98_RS15080 (H5U98_15030)	3122573..3122983	+	411	WP_077740736.1	transglycosylase family protein	-
H5U98_RS15085	3123022..3123378	+	357	WP_234815824.1	GNAT family N-acetyltransferase	-
H5U98_RS15090	3123394..3123537	+	144	WP_234815825.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-135)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 20860.95 Da
• Isoelectric Point: 7.0296

>T169714 WP_077740740.1 NZ_CP060016:c3119154-3118576 [Mycolicibacterium boenickei]
VKRLDLVVGCNGAGKSTFVELTLSPLLPGCPFVNADEIAKRRWPENPAPHSYEAALVAAETRAKLIELGESFIAETVFSH
PSKLELIDDACAAGYTVILHCILIPEDLAVQRVRHRVQAGGHDVPEAKIRERYQRLWGLVAIAAMHADSATFYDNSTVNG
PRIVAQLAGGFVVGSPTWPKWTPAVLSSRWPG

Nucleotide

• Length: 579 bp

>T169714 NZ_CP076677:c3867437-3867033 [Rhodopseudomonas palustris]
GTGACGGCTTTCTTCGATACTAACATTCTAGTCTATGCTCAGGAAACGGGCGCGAAAGCGGACCGTGCACGGGCCGTCTT
GGGCGGCGGTGGCAGGCTCAGCGTTCAAGTGTTGAACGAGTTCACCGTCGTGGTGCAGCGCAAGTTCGGCCGGGGGTGGA
GGGAGATCGCCGAGGCTATCGACGACGCTCTGGCTGTGTCCGGGCCGCCACTGCCTCTGACGCAAGATCTGCACGTTGCC
GCTCGCGTGCTCGCTGAGACGTGCAAGCTGTCGTTCTACGACGCGCTGATCGTCGCGGCCGCGCAGGAGGCCGGCTGCGA
GGTGCTGTACAGCGAGGATTTGCAGCACGGCCGCAGCTTCGGCACGTTGCGCGTCGAAAATCCGTTTCTGGAAAATCCGC
GATAA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 13778.24 Da
• Isoelectric Point: 5.2233

>AT169714 WP_077740739.1 NZ_CP060016:c3119581-3119192 [Mycolicibacterium boenickei]
MTETADRVTRFAADLMDSAAAEGARQSRSAKQQLDHWARVGRAVSSQHSAARRKVESALAGSTPLRELTAEEGAVFDAEI
AASIEENLARADYGKILAGRGITTVALDENGEIVQYRPDGTTTVLAHPQ

Nucleotide

• Length: 390 bp

>AT169714 NZ_CP076677:c3867664-3867434 [Rhodopseudomonas palustris]
ATGAAGGTCTCCAGATGGGGTAATAGCCTTGCCGTCAGGCTGCCGAATGCGCTGGTGGACAAGCTCGATCTGAAAGAGGG
CGACGAGCTCAACGTGGTCGACGTCGTCGAGCGGACGGTGGTGGTCGAGAAGGAGGACAGAAGGAAAGCCGCGCTGGAGC
GCATGGCCGAGCGCAAGTGGAAGTTGCCTGAGGGCTATCGCTTCGATCGTGACGAGGCCAACGAGCGGTGA