Detailed information of TA system TA169713

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1107996..1108628
• Replicon: chromosome
• Accession: NZ_CP060016
• Organism: Mycolicibacterium boenickei strain DSM 44677

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: H5U98_RS05430
• Protein ID: WP_077740512.1
• Coordinates: 1108179..1108628 (+)
• Length: 150 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: A0A7I7N5G7
• Locus tag: H5U98_RS05425
• Protein ID: WP_036447720.1
• Coordinates: 1107996..1108175 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5U98_RS05410 (H5U98_05375)	1103634..1104368	+	735	WP_077740509.1	enoyl-CoA hydratase	-
H5U98_RS05415 (H5U98_05380)	1104370..1105485	+	1116	WP_077740510.1	MBL fold metallo-hydrolase	-
H5U98_RS05420 (H5U98_05385)	1105552..1107927	+	2376	WP_077740511.1	cation-translocating P-type ATPase	-
H5U98_RS05425 (H5U98_05390)	1107996..1108175	+	180	WP_036447720.1	antitoxin	Antitoxin
H5U98_RS05430 (H5U98_05395)	1108179..1108628	+	450	WP_077740512.1	SRPBCC family protein	Toxin
H5U98_RS05435 (H5U98_05400)	1108683..1109042	-	360	WP_234815761.1	fumarate reductase subunit FrdD	-
H5U98_RS05440 (H5U98_05405)	1109039..1109413	-	375	WP_077740514.1	fumarate reductase subunit C	-
H5U98_RS05445 (H5U98_05410)	1109410..1110153	-	744	WP_077740515.1	succinate dehydrogenase/fumarate reductase iron-sulfur subunit	-
H5U98_RS05450 (H5U98_05415)	1110155..1111897	-	1743	WP_077740516.1	fumarate reductase (quinol) flavoprotein subunit	-
H5U98_RS05455 (H5U98_05420)	1111907..1112152	-	246	WP_234815762.1	hypothetical protein	-
H5U98_RS05460 (H5U98_05425)	1112318..1113538	-	1221	WP_077740517.1	cytochrome P450	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-145)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 150 a.a.
• Molecular weight: 16086.66 Da
• Isoelectric Point: 9.8774

>T169713 WP_077740512.1 NZ_CP060016:1108179-1108628 [Mycolicibacterium boenickei]
MAKLSVSVDVPLPPEKAWEYASDLSRYDEWLSIHRAWRSKLPETLEKGTVIDSIVEVKGMLNRVKWTLVNYKPPQSLTLN
GDGRGGVKVKLIGKIAPAQVDGGEGAKVSFDVHLGGPALFGPIGMVVAAALKGDIQQSLNKFKALYASS

Nucleotide

• Length: 450 bp

>T169713 NZ_CP076677:3590899-3591321 [Rhodopseudomonas palustris]
ATGATCGTGCTGGACACCAACGTCGTCTCCGAAGCGATGACGCGCGAACCGCATCCGCGGGTGCGCGAATGGCTCAATGC
TCAGTCCGCCGAGACGTTGTTCCTGTCCAGCATCACCGTCGCCGAACTTCTGTTCGGCGTGAGCGTCTTGCCGGCCGGAA
AGCGTAAGAACGCCTTGGGCGCTGCGCTCGACGATGTGTTGGACGTGTTTGCCACCCGCATCCTGCCGTTCGATACCCCG
GCTGCGCAGCGCTATGCCGATCTCGCCGTTAAGGCGCGCGCGGCCGGCAAGGGCTTCCCCACGCCCGACGGCTACATCGC
CGCGATCGCCACCGCGCACGGGTTCGCTGTCGCGTCGCGCGACGCGAGTGCGTTCGACGCCGCCGGTCTGACCGTGATCG
ATCCCTGGACCACAAATGACTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6392.07 Da
• Isoelectric Point: 4.4883

>AT169713 WP_036447720.1 NZ_CP060016:1107996-1108175 [Mycolicibacterium boenickei]
MGFLDKAKDLLSQNADKVEQAIDKAGDIVDEKTQGKYSGAVDKAQEAAKNAINKEEPQQ

Nucleotide

• Length: 180 bp

>AT169713 NZ_CP076677:3590632-3590886 [Rhodopseudomonas palustris]
ATGGTGGCCGTCACCATTCGCAACCTCTCCGAGGAGGCGCATCGCGCCCTGAAGGTCCGCGCGGCGCAGCACAATCGCAG
CGCGGAAGCGGAGATGCGCGCCATTCTGGAGGCCGCGGTCCGCCCCGAGGGCCGCTTGCTGCTCGGCACGGCTTTGCAGG
AGATCGGGCAGAAATACGACATCACCAATGCTGATATCGAAGCCATCGAACAGGTCCGAGACAAGCGTCCTGCCGAGCCG
ATGAAGTTTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7I7N5G7