Detailed information of TA system TA169712

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 5260529..5261155
• Replicon: chromosome
• Accession: NZ_CP060015
• Organism: Mycobacterium malmoense strain DSM 44163

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A1X0DBA4
• Locus tag: H5T25_RS24550
• Protein ID: WP_071512907.1
• Coordinates: 5260757..5261155 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: H5T25_RS24545
• Protein ID: WP_083012693.1
• Coordinates: 5260529..5260756 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5T25_RS24530 (H5T25_24360)	5256751..5258220	-	1470	WP_083012691.1	NAD(P)/FAD-dependent oxidoreductase	-
H5T25_RS24535 (H5T25_24365)	5258297..5258953	+	657	WP_071512905.1	TetR/AcrR family transcriptional regulator	-
H5T25_RS24540 (H5T25_24370)	5259262..5260467	+	1206	WP_071512910.1	DNA polymerase IV	-
H5T25_RS24545 (H5T25_24375)	5260529..5260756	+	228	WP_083012693.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
H5T25_RS24550 (H5T25_24380)	5260757..5261155	+	399	WP_071512907.1	PIN domain nuclease	Toxin
H5T25_RS24555 (H5T25_24385)	5261208..5262215	-	1008	WP_071512911.1	PHP domain-containing protein	-
H5T25_RS24560 (H5T25_24390)	5262309..5263166	+	858	WP_071512908.1	LLM class F420-dependent oxidoreductase	-
H5T25_RS24565 (H5T25_24395)	5263516..5264982	-	1467	WP_071512909.1	glutamate synthase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-119)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14977.28 Da
• Isoelectric Point: 7.2178

>T169712 WP_071512907.1 NZ_CP060015:5260757-5261155 [Mycobacterium malmoense]
MIVDTSVWIEFLTTSESLASRWLADRIAANSQVIVPEVVMMELLIGTTDESKAIRRRRLLQRFNIEPFAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVTVAHRDRDYEVIRTHCGLQTEPLF

Nucleotide

• Length: 399 bp

>T169712 NZ_CP076677:3579800-3580084 [Rhodopseudomonas palustris]
ATGATCCAGAGCTTTCGAGGCAAGTTTGCTCGGGCGATTTTGCAGGACCGGAAAGCCCCCAAGGGCCTTCCGGCCGACCT
CCTGGGCACTGCCCGGCGCAAATTGGTTCAGCTCAACGCCGCCGCTGCTCTGGCCGACCTTGCGATCCCTCCCGGCAATC
GCCTGGAAGCCCTACGCGGAGATTTGCAGGGTCTCCATTCAATCCGGATCAACGATCAGTGGCGGATCGTGTTTCGCTGG
AAAGACACTGGTCCGGAAGACGTCGAGATCGTCGACTATCACTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8334.16 Da
• Isoelectric Point: 4.0057

>AT169712 WP_083012693.1 NZ_CP060015:5260529-5260756 [Mycobacterium malmoense]
MSRTNIEINDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLDRDEALALQGSGFDFTNDEIESFSDTGIDLADQS

Nucleotide

• Length: 228 bp

>AT169712 NZ_CP076677:3580097-3580399 [Rhodopseudomonas palustris]
ATGACGAAGAAACTATCCCCTGTTCACCCCGGCGAAATTCTCCGCGAGGAGTTTCTGCTGCCGATGAACCTGTCGCCCTA
CGCCGTCGCCGCAGCCTGCGGCGTACCGCGCACGCGCATCGAACGGCTGGCACGGCAGGAAACCCCAGTAACGGCGGACA
CCGCGCTGCGGCTGGCGAAATACTTCGGCACGACGCCGAATTTCTGGATGAACATCCAAGCGCAGTACGATCTGGAGGTC
GCGGAAGACAACAGCGCCGACGAACTAAGACGGATCAAGCCAATCAAGTCGAGAGCGGCCTAA

Structures

Toxin

• AlphaFold DB: A0A1X0DBA4