Detailed information of TA system TA169711

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 4440765..4441408
• Replicon: chromosome
• Accession: NZ_CP060015
• Organism: Mycobacterium malmoense strain DSM 44163

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H5T25_RS20510
• Protein ID: WP_071509157.1
• Coordinates: 4440765..4441154 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: H5T25_RS20515
• Protein ID: WP_083011961.1
• Coordinates: 4441151..4441408 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5T25_RS20475 (H5T25_20330)	4436664..4436945	-	282	WP_139797163.1	hypothetical protein	-
H5T25_RS20480 (H5T25_20335)	4437229..4437768	+	540	WP_139797162.1	hypothetical protein	-
H5T25_RS20485 (H5T25_20340)	4437780..4438001	+	222	WP_071508759.1	DUF4926 domain-containing protein	-
H5T25_RS20490 (H5T25_20345)	4438107..4438351	-	245	Protein_4067	SAM-dependent methyltransferase	-
H5T25_RS20495 (H5T25_20350)	4439088..4439552	-	465	Protein_4068	hypothetical protein	-
H5T25_RS20500 (H5T25_20355)	4439918..4440205	+	288	WP_176220053.1	DUF1778 domain-containing protein	-
H5T25_RS20505 (H5T25_20360)	4440458..4440778	+	321	WP_083011963.1	GNAT family N-acetyltransferase	-
H5T25_RS20510 (H5T25_20365)	4440765..4441154	-	390	WP_071509157.1	type II toxin-antitoxin system VapC family toxin	Toxin
H5T25_RS20515 (H5T25_20370)	4441151..4441408	-	258	WP_083011961.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
H5T25_RS20520 (H5T25_20375)	4441558..4442280	+	723	WP_139797161.1	hypothetical protein	-
H5T25_RS20525 (H5T25_20380)	4442273..4443052	+	780	WP_083011956.1	hypothetical protein	-
H5T25_RS20530 (H5T25_20385)	4443064..4443314	-	251	Protein_4075	hypothetical protein	-
H5T25_RS20535 (H5T25_20390)	4443646..4444587	-	942	WP_083011953.1	class I SAM-dependent methyltransferase	-
H5T25_RS20540 (H5T25_20395)	4444590..4445531	-	942	WP_083012006.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4437442..4454903	17461

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-121)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (4-50)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14085.00 Da
• Isoelectric Point: 4.5242

>T169711 WP_071509157.1 NZ_CP060015:c4441154-4440765 [Mycobacterium malmoense]
VMVRRALADTSIFIGIEAARFDDDRFTEHEWGVSVITLGELRLGVLQASDPDTAARRLSTYQLAQRFEPLSIDETVSEAW
ALLVSKRAANLRAPINDSWIAATAMAHGIPIVTQGSDYSAMPGVEVITI

Nucleotide

• Length: 390 bp

>T169711 NZ_CP076676:4628432-4628710 [Rhodopseudomonas palustris]
ATGATCCGCTCGTTCCGAAATCCGGCGACCGCGCGGCTGTTTGCTGATGAGGACGTGCCGCGGTTTCGCGCGATCGAACG
GCAAGCTCGTCGAAAGCTGCTGCTGCTGGACGGCGCCGCCAAACTCGACGATTTGCGGCAACCTCCGGGCAACAGGCTCG
AGGCCTTGAAGGGCGATCGCCGCGGCCAATACTCGATCCGCATCAACGATCAATGGCGGATTTGTTTCGAGTGGCGTGAG
GACGGCGCAGAAATGGTCGAAATCGTGGACTATCATTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9678.07 Da
• Isoelectric Point: 6.3620

>AT169711 WP_083011961.1 NZ_CP060015:c4441408-4441151 [Mycobacterium malmoense]
MAATIPARDLRNRTAEVLRRVAAGEEIEVLKDNRPVARIVPLTRRRQWLPAAEVIGELMRLGPDTTNLSEELRETLTRTT
DDVSW

Nucleotide

• Length: 258 bp

>AT169711 NZ_CP076676:4628722-4629027 [Rhodopseudomonas palustris]
ATGGCCAAACCAAAGCAGCTCCCCCCGATCCCTCCCGGCGAGATTCTGATCGAGGAATTCATGCGCCCGAACGGGATCAG
TCAGAACCGGCTGGCGCGCGATATCGATATCAATCCGGCCCGGGTCAACGACATCGTGCACGGCCGCTCTGCGATCACCG
CCGCCGTCGCCCTGCGGCTGGCGAAGTACTTCGGCACGACGCCCGAACTCTGGATGAACCTGCAGGCGTCCTACGACCTG
CGCCGTGCCCGCGCCGCAGACTGGCCCGCGATTGAGCCGCGCGTCCGCGTGCTCGCCGCGGAGTAG