Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2881063..2881748 Replicon chromosome
Accession NZ_CP060015
Organism Mycobacterium malmoense strain DSM 44163

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag H5T25_RS13525 Protein ID WP_083011068.1
Coordinates 2881063..2881494 (-) Length 144 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag H5T25_RS13530 Protein ID WP_083011067.1
Coordinates 2881491..2881748 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5T25_RS13495 (H5T25_13385) 2876769..2877242 - 474 WP_230981213.1 type II toxin-antitoxin system VapC family toxin -
H5T25_RS13500 (H5T25_13390) 2877181..2877414 - 234 WP_230981214.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
H5T25_RS13505 (H5T25_13395) 2877930..2878844 + 915 WP_230981217.1 SAM-dependent methyltransferase -
H5T25_RS13510 (H5T25_13400) 2878914..2879564 - 651 WP_071510324.1 TetR/AcrR family transcriptional regulator -
H5T25_RS13515 (H5T25_13405) 2880090..2880530 + 441 WP_139797034.1 hypothetical protein -
H5T25_RS13520 (H5T25_13410) 2880485..2880886 + 402 WP_277622357.1 DUF5615 family PIN-like protein -
H5T25_RS13525 (H5T25_13415) 2881063..2881494 - 432 WP_083011068.1 type II toxin-antitoxin system VapC family toxin Toxin
H5T25_RS13530 (H5T25_13420) 2881491..2881748 - 258 WP_083011067.1 CopG family transcriptional regulator Antitoxin
H5T25_RS13535 (H5T25_13425) 2882088..2882774 - 687 Protein_2690 alpha/beta hydrolase -
H5T25_RS13540 (H5T25_13430) 2883139..2884620 + 1482 WP_139797033.1 biotin carboxylase -
H5T25_RS13545 (H5T25_13435) 2884664..2885860 + 1197 WP_083011066.1 serine hydrolase -
H5T25_RS13550 (H5T25_13440) 2886370..2886629 + 260 Protein_2693 antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2850288..2881748 31460


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-133)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-36)


Sequences

Toxin        


Download         Length: 144 a.a.        Molecular weight: 15770.86 Da        Isoelectric Point: 4.5713

>T169703 WP_083011068.1 NZ_CP060015:c2881494-2881063 [Mycobacterium malmoense]
MRLLDLNILIYAIDESSSRHVVAKDWLDDTLSGSDTVAFSWHVLIGFVRLSTRAAVFERPLTVDESFDVVDGWLEQPCVT
VVHPTDRHAIVLRGILTPLGTAGNLTSDAHLAALSIEHGAELCSTDVDFSRFSGVRWIDPLQT

Download         Length: 432 bp

>T169703 NZ_CP076668:c5000554-5000270 [Pseudomonas lijiangensis]
ATGCGCGTTGAGTGGCTGAGGACAGCGCTGAAGAATCTCGATGACGAAGCCGCTTACATCGCCGTGGATAATCCGCAGTC
TGCCGTTGAGTTCGTGCTCGCCATAAGGGCTGGCGTAGAACAACTGGCTCTTTTTCCTGCGATGGGGCGAGAAGGGCGAC
TCAGTGGTACTCGTGAATGGGTGTTGCCTGATCGGCCGTACATCATTCCTTATCGTGTTCGCGGCGGATGTTTGCAGATA
TTGCGCATTTTCCACACCCGCCGTTTACCGCCATCAAGCTGGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9348.72 Da        Isoelectric Point: 11.0899

>AT169703 WP_083011067.1 NZ_CP060015:c2881748-2881491 [Mycobacterium malmoense]
MRTTVTLDPDVVAALQRAARERGASFKAVLNDAVRRGLSGGPTQRRYHTPSRDMGLRTGFDIDKALTLVAADEDAEILRK
LALRK

Download         Length: 258 bp

>AT169703 NZ_CP076668:c5000783-5000544 [Pseudomonas lijiangensis]
ATGTCCACTATGTCTATACGTATACCTGATGAAATGGCGGCCACCCTTGCCAGCCTTGCAAAAGCTACCGGGCGCACCAA
GTCGTTCCTGGCCATTGATGCCTTGCGTGAATACCTCGATCGTGAGGCCTGGCAGATTGCCGAGATTCAGCGCTCGATCC
AGGAGGCGGATGCCGGGGATTTTGCCAGCGATGAAGAGGTCGGCGCCGTGATGGACAAGTGGACAGGCAATGCGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References