Detailed information of TA system TA169702

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 2870662..2871197
• Replicon: chromosome
• Accession: NZ_CP060015
• Organism: Mycobacterium malmoense strain DSM 44163

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: H5T25_RS13475
• Protein ID: WP_071510758.1
• Coordinates: 2870865..2871197 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: H5T25_RS13470
• Protein ID: WP_071510757.1
• Coordinates: 2870662..2870868 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5T25_RS13425 (H5T25_13315)	2865710..2865916	+	207	WP_071510751.1	DUF2191 domain-containing protein	-
H5T25_RS13430 (H5T25_13320)	2865907..2866329	+	423	WP_083011075.1	PIN domain-containing protein	-
H5T25_RS13435 (H5T25_13325)	2866454..2867284	-	831	WP_071510753.1	DUF1906 domain-containing protein	-
H5T25_RS13440 (H5T25_13330)	2867761..2867997	+	237	WP_083011074.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
H5T25_RS13445 (H5T25_13335)	2868018..2868431	+	414	WP_071510773.1	type II toxin-antitoxin system VapC family toxin	-
H5T25_RS13450 (H5T25_13340)	2868713..2868946	+	234	WP_071510754.1	hypothetical protein	-
H5T25_RS13455 (H5T25_13345)	2868936..2869247	+	312	WP_071510755.1	type II toxin-antitoxin system PemK/MazF family toxin	-
H5T25_RS13460 (H5T25_13350)	2869560..2870018	+	459	WP_083011073.1	prevent-host-death protein	-
H5T25_RS13465 (H5T25_13355)	2870030..2870392	+	363	WP_083011072.1	hypothetical protein	-
H5T25_RS13470 (H5T25_13360)	2870662..2870868	+	207	WP_071510757.1	DUF3018 family protein	Antitoxin
H5T25_RS13475 (H5T25_13365)	2870865..2871197	+	333	WP_071510758.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
H5T25_RS13480 (H5T25_13370)	2871440..2873041	-	1602	WP_071510805.1	integrase	-
H5T25_RS13485 (H5T25_13375)	2873357..2874358	-	1002	WP_265415707.1	hypothetical protein	-
H5T25_RS13490 (H5T25_13380)	2874262..2876097	-	1836	WP_265415708.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2850288..2881748	31460

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

• Antitoxin MazE-like (4-66)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11803.62 Da
• Isoelectric Point: 7.2039

>T169702 WP_071510758.1 NZ_CP060015:2870865-2871197 [Mycobacterium malmoense]
VNRGEIWTVAGGVYAAKPRPAVIVQDDLFDATSSVTVAPMSSTLLDAPLMRIRIAGGDGRLSGLDHDSDVMIDKLTTVKR
SNVHARVGRLTAEQVVEVERAMMAFLGLAR

Nucleotide

• Length: 333 bp

>T169702 NZ_CP076668:c4836694-4836512 [Pseudomonas lijiangensis]
ATGCGAAGTCGTGATTTGATTGAAATGATTGAGGCGGATGGATGGTTTGAAGTGGCGGTGAAAGGAAGCCATCATCAGTT
CAAACATCCAACCAAAAAAGGGCGGGTGACTGTTCCACACCCCAAGTCTGAAATAGCTAAAGGAACGCTACATAACATAT
TGAAAAGCGCCGGCCTCAAATGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7915.77 Da
• Isoelectric Point: 4.4805

>AT169702 WP_071510757.1 NZ_CP060015:2870662-2870868 [Mycobacterium malmoense]
MAVRERVGEYRRRMRERGLRPLQVWVPDVRTESFAAEAHRQASLVALADESADDQDFIEAISTPWDEE

Nucleotide

• Length: 207 bp

>AT169702 NZ_CP076668:c4836363-4835962 [Pseudomonas lijiangensis]
ATGAAATTTCCAGTCGTAATACATAAAGACGCCGACTCTGATTTTAGTGTGACTGTCCCCGATGTGCCTGGATGCTTCTC
GGCCGGTGCCACTTTCTCGCAAGCGCTTGAAAATGTACACGAAGCACTGGCGCTGCATCTGGAGGGGCTCGTAGCTGATG
ATGAGGTTTTACCTCAGGCGCAGGAAGTAGACGCGCACCTCGGTAATCCGGACTACGCAGATGGCATATGGGCTTTGGTC
GACTTCGACTTGACCCCTTATCTGGGGAAGTCGGTGAGGTTTAACGCCTCTCTGCCGGAACATCTTCTGAACAGGATCGA
TGAACGAGTTCAGAAGGATCACCGCTACGCTTCGCGATCAGGCTTTTTGGCAACTGCGGCGTTGAGAGAGTTGTGTGCCT
GA