Detailed information of TA system TA169700

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2245500..2246128
• Replicon: chromosome
• Accession: NZ_CP060015
• Organism: Mycobacterium malmoense strain DSM 44163

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H5T25_RS10620
• Protein ID: WP_083011642.1
• Coordinates: 2245715..2246128 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: H5T25_RS10615
• Protein ID: WP_071510089.1
• Coordinates: 2245500..2245724 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5T25_RS10585 (H5T25_10505)	2240738..2241109	-	372	WP_083010903.1	hypothetical protein	-
H5T25_RS10590 (H5T25_10510)	2241222..2241872	-	651	WP_139797002.1	hypothetical protein	-
H5T25_RS10595 (H5T25_10515)	2242278..2242838	-	561	WP_071513463.1	MarR family transcriptional regulator	-
H5T25_RS10600 (H5T25_10520)	2243732..2244031	+	300	WP_220688608.1	PE family protein	-
H5T25_RS10605	2244089..2244465	+	377	Protein_2106	PPE family protein	-
H5T25_RS10610	2244931..2245266	+	336	WP_265415743.1	hypothetical protein	-
H5T25_RS10615 (H5T25_10530)	2245500..2245724	+	225	WP_071510089.1	hypothetical protein	Antitoxin
H5T25_RS10620 (H5T25_10535)	2245715..2246128	+	414	WP_083011642.1	PIN domain nuclease	Toxin
H5T25_RS10625 (H5T25_10540)	2246990..2247565	+	576	Protein_2110	PPE family protein	-
H5T25_RS10630 (H5T25_10545)	2247793..2248173	+	381	WP_265415740.1	PE/PPE C-terminal domain-containing protein	-
H5T25_RS10635 (H5T25_10550)	2248255..2249055	+	801	WP_230981670.1	sulfotransferase	-
H5T25_RS10640 (H5T25_10555)	2249544..2249876	+	333	WP_139797137.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2241222..2289692	48470

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (24-126)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14950.17 Da
• Isoelectric Point: 8.5681

>T169700 WP_083011642.1 NZ_CP060015:2245715-2246128 [Mycobacterium malmoense]
VAVTRWLIDKSAYARLQLGQASNVDQWNARIGRGLVHLSTITRLELGYSARNGHAGRRQFASPPLSLMPVEYLTPAIEDR
ALEVQMLLADRGQHRAPSIPDLLVAATAEKAGLRVLVADKDFDLIANITGQPIDALT

Nucleotide

• Length: 414 bp

>T169700 NZ_CP076660:c2197392-2197126 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGATCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8176.19 Da
• Isoelectric Point: 4.3867

>AT169700 WP_071510089.1 NZ_CP060015:2245500-2245724 [Mycobacterium malmoense]
MPDVLIRGLSEEAVENIDAAAAALGLSRNEYLRRTFERAAVQPVKKTVTAEDWQRSAETFADLADPDVMAAAWR

Nucleotide

• Length: 225 bp

>AT169700 NZ_CP076660:c2197649-2197392 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAGTAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTGTAA