Detailed information of TA system TA169699

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PhRel-ATphRel/-
• Location: 2151633..2152695
• Replicon: chromosome
• Accession: NZ_CP060015
• Organism: Mycobacterium malmoense strain DSM 44163

Toxin (Protein)

• Gene name: phRel
• Uniprot ID: -
• Locus tag: H5T25_RS10200
• Protein ID: WP_071510778.1
• Coordinates: 2151865..2152695 (-)
• Length: 277 a.a.

Antitoxin (Protein)

• Gene name: ATphRel
• Uniprot ID: -
• Locus tag: H5T25_RS10195
• Protein ID: WP_230981627.1
• Coordinates: 2151633..2151863 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5T25_RS10175 (H5T25_10095)	2147673..2148011	-	339	WP_054585837.1	hypothetical protein	-
H5T25_RS10180 (H5T25_10100)	2148300..2148641	+	342	WP_230981626.1	antibiotic biosynthesis monooxygenase	-
H5T25_RS10185 (H5T25_10105)	2148628..2149845	+	1218	WP_071510779.1	helix-turn-helix domain-containing protein	-
H5T25_RS10190 (H5T25_10110)	2150458..2151126	-	669	WP_265415739.1	class I SAM-dependent methyltransferase	-
H5T25_RS10195 (H5T25_10120)	2151633..2151863	-	231	WP_230981627.1	hypothetical protein	Antitoxin
H5T25_RS10200 (H5T25_10125)	2151865..2152695	-	831	WP_071510778.1	hypothetical protein	Toxin
H5T25_RS10205 (H5T25_10130)	2152853..2153230	+	378	Protein_2026	hypothetical protein	-
H5T25_RS10210 (H5T25_10135)	2153250..2155193	+	1944	WP_083010857.1	excinuclease ABC subunit UvrC	-
H5T25_RS10215 (H5T25_10140)	2155190..2156077	+	888	WP_071513530.1	RNase adapter RapZ	-
H5T25_RS10220 (H5T25_10145)	2156074..2157106	+	1033	Protein_2029	uridine diphosphate-N-acetylglucosamine-binding protein YvcK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RelA/SpoT (67-177)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 277 a.a.
• Molecular weight: 30202.93 Da
• Isoelectric Point: 4.6221

>T169699 WP_071510778.1 NZ_CP060015:c2152695-2151865 [Mycobacterium malmoense]
VDLESRPPWSNNALRRLGAALRDGVDPPIDCPSYADVMLWHFDLAAEVQDQIESGSWSIRSELLATKAGRLTPDLQVSSR
PKTQDTLVEKLQRQPSLQLNAVQDLAGVRIDADLLLGEQMSLAREIASYFCADESAIHDLRDGTHAGYRGVHVWLRLPAG
RVEIQIRTILQSLWANLFEKLADEVGRGIRYGEPVEPSPGVDVGRIQRAVQSMQEFSAKVAASEADWQQCSEIEDPGRRG
VALGTAAMDRAMWVGLVAYAAQKASGEAESSKGEDG

Nucleotide

• Length: 831 bp

>T169699 NZ_CP076660:c2165995-2165888 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8845.01 Da
• Isoelectric Point: 6.4636

>AT169699 WP_230981627.1 NZ_CP060015:c2151863-2151633 [Mycobacterium malmoense]
MAGYVVEYNRRTHERRITEFVTTREAMEYRLKLEAERTDKNVEIAALVSKSLDTLKQTHSRYFSGAELAAEDCMLT

Nucleotide

• Length: 231 bp

>AT169699 NZ_CP076660:2165811-2165871 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA