Detailed information of TA system TA169696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 829005..829570
• Replicon: chromosome
• Accession: NZ_CP060015
• Organism: Mycobacterium malmoense strain DSM 44163

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: H5T25_RS03885
• Protein ID: WP_277622396.1
• Coordinates: 829005..829322 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: H5T25_RS03890
• Protein ID: WP_277622374.1
• Coordinates: 829337..829570 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5T25_RS03860 (H5T25_03830)	824372..825619	-	1248	WP_083009269.1	FAD-dependent monooxygenase	-
H5T25_RS03865 (H5T25_03835)	825695..826231	+	537	WP_083009272.1	TetR/AcrR family transcriptional regulator	-
H5T25_RS03870 (H5T25_03840)	826261..827250	+	990	WP_083009274.1	LLM class F420-dependent oxidoreductase	-
H5T25_RS03875 (H5T25_03845)	827334..827729	-	396	WP_230981573.1	hypothetical protein	-
H5T25_RS03880 (H5T25_03850)	828055..828957	+	903	WP_083009277.1	DMT family transporter	-
H5T25_RS03885 (H5T25_03855)	829005..829322	-	318	WP_277622396.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
H5T25_RS03890 (H5T25_03860)	829337..829570	-	234	WP_277622374.1	antitoxin	Antitoxin
H5T25_RS03895 (H5T25_03865)	829989..830264	-	276	WP_265415728.1	hypothetical protein	-
H5T25_RS03900 (H5T25_03870)	830328..830594	+	267	WP_139796754.1	hypothetical protein	-
H5T25_RS03905 (H5T25_03875)	830819..831037	-	219	WP_083009286.1	antitoxin	-
H5T25_RS03910 (H5T25_03880)	831476..831835	+	360	WP_230981574.1	type II toxin-antitoxin system VapC family toxin	-
H5T25_RS03915 (H5T25_03885)	831768..832205	-	438	WP_254893541.1	helix-turn-helix transcriptional regulator	-
H5T25_RS03920 (H5T25_03890)	832857..834035	+	1179	WP_083009288.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	827759..842403	14644

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-104)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11253.97 Da
• Isoelectric Point: 11.6144

>T169696 WP_277622396.1 NZ_CP060015:c829322-829005 [Mycobacterium malmoense]
IFIVSLDPTQGHEQRGTRPVLIVSPTAFNRLTQTPVVLPITRGGNFARTAGFAVSLTDAGTRTTGVIRCDQPRAIDIGSR
NGRKVERVPAAILDEVLAKLATLFS

Nucleotide

• Length: 318 bp

>T169696 NZ_CP076660:c1803762-1803400 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACATACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGTTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8109.19 Da
• Isoelectric Point: 3.9500

>AT169696 WP_277622374.1 NZ_CP060015:c829570-829337 [Mycobacterium malmoense]
VGGSVMLAVPPAVLDMLRLHAGSAVGLSVDAGHLIVEPQTRPRYTLDELLSQCDASADLTDEDRVWLDGEPVGGELL

Nucleotide

• Length: 234 bp

>AT169696 NZ_CP076660:c1803929-1803759 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA