Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 829005..829570 Replicon chromosome
Accession NZ_CP060015
Organism Mycobacterium malmoense strain DSM 44163

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag H5T25_RS03885 Protein ID WP_277622396.1
Coordinates 829005..829322 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID -
Locus tag H5T25_RS03890 Protein ID WP_277622374.1
Coordinates 829337..829570 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5T25_RS03860 (H5T25_03830) 824372..825619 - 1248 WP_083009269.1 FAD-dependent monooxygenase -
H5T25_RS03865 (H5T25_03835) 825695..826231 + 537 WP_083009272.1 TetR/AcrR family transcriptional regulator -
H5T25_RS03870 (H5T25_03840) 826261..827250 + 990 WP_083009274.1 LLM class F420-dependent oxidoreductase -
H5T25_RS03875 (H5T25_03845) 827334..827729 - 396 WP_230981573.1 hypothetical protein -
H5T25_RS03880 (H5T25_03850) 828055..828957 + 903 WP_083009277.1 DMT family transporter -
H5T25_RS03885 (H5T25_03855) 829005..829322 - 318 WP_277622396.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
H5T25_RS03890 (H5T25_03860) 829337..829570 - 234 WP_277622374.1 antitoxin Antitoxin
H5T25_RS03895 (H5T25_03865) 829989..830264 - 276 WP_265415728.1 hypothetical protein -
H5T25_RS03900 (H5T25_03870) 830328..830594 + 267 WP_139796754.1 hypothetical protein -
H5T25_RS03905 (H5T25_03875) 830819..831037 - 219 WP_083009286.1 antitoxin -
H5T25_RS03910 (H5T25_03880) 831476..831835 + 360 WP_230981574.1 type II toxin-antitoxin system VapC family toxin -
H5T25_RS03915 (H5T25_03885) 831768..832205 - 438 WP_254893541.1 helix-turn-helix transcriptional regulator -
H5T25_RS03920 (H5T25_03890) 832857..834035 + 1179 WP_083009288.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 827759..842403 14644


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11253.97 Da        Isoelectric Point: 11.6144

>T169696 WP_277622396.1 NZ_CP060015:c829322-829005 [Mycobacterium malmoense]
IFIVSLDPTQGHEQRGTRPVLIVSPTAFNRLTQTPVVLPITRGGNFARTAGFAVSLTDAGTRTTGVIRCDQPRAIDIGSR
NGRKVERVPAAILDEVLAKLATLFS

Download         Length: 318 bp

>T169696 NZ_CP076660:c1803762-1803400 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACATACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGTTCATCAGAAAAATTAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8109.19 Da        Isoelectric Point: 3.9500

>AT169696 WP_277622374.1 NZ_CP060015:c829570-829337 [Mycobacterium malmoense]
VGGSVMLAVPPAVLDMLRLHAGSAVGLSVDAGHLIVEPQTRPRYTLDELLSQCDASADLTDEDRVWLDGEPVGGELL

Download         Length: 234 bp

>AT169696 NZ_CP076660:c1803929-1803759 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References