Detailed information of TA system TA169694

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): abiEi-abiEii/AbiEii-AbiEi
• Location: 572209..574010
• Replicon: chromosome
• Accession: NZ_CP060015
• Organism: Mycobacterium malmoense strain DSM 44163

Toxin (Protein)

• Gene name: abiEi
• Uniprot ID: -
• Locus tag: H5T25_RS02640
• Protein ID: WP_139797173.1
• Coordinates: 573126..574010 (+)
• Length: 295 a.a.

Antitoxin (Protein)

• Gene name: abiEii
• Uniprot ID: -
• Locus tag: H5T25_RS02635
• Protein ID: WP_083012113.1
• Coordinates: 572209..573123 (+)
• Length: 305 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5T25_RS02615 (H5T25_02590)	568822..569505	+	684	WP_083012175.1	GAP family protein	-
H5T25_RS02620 (H5T25_02595)	569562..570233	+	672	WP_083012108.1	GAP family protein	-
H5T25_RS02625 (H5T25_02600)	570209..571033	-	825	WP_230981568.1	hypothetical protein	-
H5T25_RS02630 (H5T25_02605)	571030..571368	-	339	WP_083012110.1	DUF732 domain-containing protein	-
H5T25_RS02635 (H5T25_02610)	572209..573123	+	915	WP_083012113.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
H5T25_RS02640 (H5T25_02615)	573126..574010	+	885	WP_139797173.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
H5T25_RS02650 (H5T25_02625)	574341..574913	+	573	WP_071508660.1	dCTP deaminase	-
H5T25_RS02655 (H5T25_02630)	575037..576848	+	1812	WP_230981569.1	hypothetical protein	-
H5T25_RS02660 (H5T25_02635)	577136..578695	+	1560	WP_083012120.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (39-267)

Antitoxin

• AbiEi antitoxin C-terminal domain (91-243)

Sequences

Toxin

Protein

• Length: 295 a.a.
• Molecular weight: 32495.02 Da
• Isoelectric Point: 6.6085

>T169694 WP_139797173.1 NZ_CP060015:573126-574010 [Mycobacterium malmoense]
VAGLTRALVARHAAGRADAYDAALLDVAQDHLLFLLAQAANIGDGRLVFKGGTSLRKCRLGNAGRFSIDLDFGAPDDDVV
LEVCGLIDGARIGGFEFGIVSTRGDGRHWGLTVSHAELGAPRIAASVEFARRPLALPPEPLPFVPLPIHRAYGFPLPTLP
VVAEAEACAEKLARYRRVALARDLYDLNHFATRTFDEPLVRRLWVLKVWADVVDDRRGGRPLRPEDILTVRGEQDFEPDS
IGILTRPVDIPDWEARVRRRFAFLTDLDANEQRWASCNERHRSEVQDALSALRG

Nucleotide

• Length: 885 bp

>T169694 NZ_CP076660:c1607799-1607647 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 305 a.a.
• Molecular weight: 33047.30 Da
• Isoelectric Point: 9.9380

>AT169694 WP_083012113.1 NZ_CP060015:572209-573123 [Mycobacterium malmoense]
MNLSAHGATSPLGADRRIPKWASAVIGGLARDRPIVVTTEDLALRLAEATSERDVDLAIRDLRRLGWLVGLPVRGTWAFI
PPGEAGVSDPYLPVRAWLARDHDAGCMLAGAAAAWHLGYLDRQPQGRVSLWLPAKTRLPDGLRKYVSIVKISWNIQDAAQ
LAPSTALLLRRRLDIVSWATGLPAFGPEALLVQLAARPSSFRPWADIVAHLDVLAADCVDEHLLPLLSGQSMSTWQRASY
LIDVAGDPERGAALLAKRQMKTMSVVQFTHPHIRASRSTEGVWVPKYQLVDGLVAPLLSVVGKA

Nucleotide

• Length: 915 bp

>AT169694 NZ_CP076660:c1608423-1607824 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACTGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATATTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTTGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
CGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA