Detailed information of TA system TA169693

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 111011..111621
• Replicon: chromosome
• Accession: NZ_CP060015
• Organism: Mycobacterium malmoense strain DSM 44163

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H5T25_RS00560
• Protein ID: WP_083008796.1
• Coordinates: 111011..111403 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: H5T25_RS00565
• Protein ID: WP_083008799.1
• Coordinates: 111400..111621 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5T25_RS00535 (H5T25_00535)	106638..107243	+	606	WP_139796726.1	class I SAM-dependent methyltransferase	-
H5T25_RS00540 (H5T25_00540)	107356..107805	+	450	WP_083009126.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
H5T25_RS00545 (H5T25_00545)	107959..108633	-	675	WP_230981547.1	TetR/AcrR family transcriptional regulator	-
H5T25_RS00550 (H5T25_00550)	108703..110400	+	1698	WP_176219862.1	FAD-dependent monooxygenase	-
H5T25_RS00555 (H5T25_00555)	110418..110798	-	381	WP_230981548.1	hypothetical protein	-
H5T25_RS00560 (H5T25_00560)	111011..111403	-	393	WP_083008796.1	PIN domain-containing protein	Toxin
H5T25_RS00565 (H5T25_00565)	111400..111621	-	222	WP_083008799.1	CopG family transcriptional regulator	Antitoxin
H5T25_RS00570 (H5T25_00570)	111704..113046	-	1343	Protein_110	aromatic ring-hydroxylating dioxygenase subunit alpha	-
H5T25_RS00575 (H5T25_00575)	113058..114830	-	1773	WP_083008804.1	amidohydrolase family protein	-
H5T25_RS00580 (H5T25_00580)	115010..116158	-	1149	WP_083008807.1	phosphotransferase	-
H5T25_RS00585 (H5T25_00585)	116181..116558	-	378	WP_083009128.1	STAS domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-125)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-39)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14877.74 Da
• Isoelectric Point: 6.3775

>T169693 WP_083008796.1 NZ_CP060015:c111403-111011 [Mycobacterium malmoense]
MIFVDTSFWAALGNATDAHHTAAERLWAAKPPFVVTSNHVLGETWTLLNRRCGHRVAVTAAAIRYSSHVRLEHITAELEE
EAWEWLARHDEREYSFVDATSFALMRKKKLRDAYAFDEDFSAAGFVELRP

Nucleotide

• Length: 393 bp

>T169693 NZ_CP076660:1553200-1553295 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGTGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8147.17 Da
• Isoelectric Point: 4.7683

>AT169693 WP_083008799.1 NZ_CP060015:c111621-111400 [Mycobacterium malmoense]
MKRLQIYIEEDLDHALGIEARRQRKSKAALIRAYVADRLGAREPDPIDAFIGSFDGGVDLSTSVDDVVYGPRE

Nucleotide

• Length: 222 bp

>AT169693 NZ_CP076660:c1553380-1553323 [Staphylococcus aureus]
AACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG