Detailed information of TA system TA169686

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 1681619..1683021
• Replicon: chromosome
• Accession: NZ_CP060009
• Organism: Pseudomonas sediminis strain B10D7D

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HNQ25_RS07990
• Protein ID: WP_179544247.1
• Coordinates: 1681619..1682188 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HNQ25_RS07995
• Protein ID: WP_179544248.1
• Coordinates: 1682194..1683021 (+)
• Length: 276 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HNQ25_RS07985	1677925..1681413	-	3489	WP_179544246.1	hybrid sensor histidine kinase/response regulator	-
HNQ25_RS07990	1681619..1682188	+	570	WP_179544247.1	GNAT family N-acetyltransferase	Toxin
HNQ25_RS07995	1682194..1683021	+	828	WP_179544248.1	AraC family transcriptional regulator	Antitoxin
HNQ25_RS08000	1683164..1684501	-	1338	WP_179544249.1	peptidase	-
HNQ25_RS08005	1684692..1685264	+	573	WP_179544250.1	LemA family protein	-
HNQ25_RS08010	1685408..1686313	+	906	WP_179544251.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (14-146)

Antitoxin

• DNA binding HTH domain, AraC-type (193-270)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21466.66 Da
• Isoelectric Point: 7.3597

>T169686 WP_179544247.1 NZ_CP060009:1681619-1682188 [Pseudomonas sediminis]
MFKPQLVTLQRGALRLEPLSDADIPGLVALAEANREELLYMSGTQRLDWYRSALADLREQRALPFVIRLGDEMVGTTRFA
DFMNTLPACEIGWTWLDHRQHGSGLNASIKYLMLKHAFENWGMVRVQLKTAASNLRSQRAIEKLGAVREGVLRNHRRLAD
GRLDDTVLYSITDLEWPALREAMEAGFNA

Nucleotide

• Length: 570 bp

>T169686 NZ_CP076655:68849-68998 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Protein

• Length: 276 a.a.
• Molecular weight: 30908.29 Da
• Isoelectric Point: 8.7343

>AT169686 WP_179544248.1 NZ_CP060009:1682194-1683021 [Pseudomonas sediminis]
MHRREQTRFWQASALSGVELLQARYLEQRFAPHVHEGFVFTVIEHGAQRFRHRGCDHLAPQGSMVLINPDEVHTGSKAHE
DGWRYRGFYPDNAQVLGALRELGLAKAGMPSFAFSVLDDPRLHAAFLQLHQLLEHGTEALQQQLAWREAILLLFQHHAQL
AEPPAPGREPWAVACATQMLAERLVEPPSLEELAAAVNLSPFHFARVFRRATGLPPHAWLKQRRLEQARALLKTGCTAVA
VAMQLGFADQSHLSRQFKQVYGVSPGEYRSAVARS

Nucleotide

• Length: 828 bp

>AT169686 NZ_CP076655:c68805-68744 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT