Detailed information of TA system TA169683

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 3775898..3776484
• Replicon: chromosome
• Accession: NZ_CP060008
• Organism: Pseudomonas sp. B11D7D

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: HNQ27_RS17220
• Protein ID: WP_179573151.1
• Coordinates: 3775898..3776191 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A7G7RNS1
• Locus tag: HNQ27_RS17225
• Protein ID: WP_074937747.1
• Coordinates: 3776194..3776484 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HNQ27_RS17200	3772002..3772424	+	423	WP_179573148.1	VOC family protein	-
HNQ27_RS17205	3772473..3773312	-	840	WP_036999484.1	UTP--glucose-1-phosphate uridylyltransferase GalU	-
HNQ27_RS17210	3773355..3774716	-	1362	WP_179573149.1	UDP-glucose/GDP-mannose dehydrogenase family protein	-
HNQ27_RS17215	3774893..3775561	+	669	WP_179573150.1	hypothetical protein	-
HNQ27_RS17220	3775898..3776191	+	294	WP_179573151.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HNQ27_RS17225	3776194..3776484	+	291	WP_074937747.1	putative addiction module antidote protein	Antitoxin
HNQ27_RS17245	3777251..3777811	-	561	WP_036999480.1	CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase	-
HNQ27_RS17250	3777845..3779668	-	1824	WP_074937732.1	excinuclease ABC subunit UvrC	-
HNQ27_RS17255	3779671..3780315	-	645	WP_036999477.1	UvrY/SirA/GacA family response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3775898..3822839	46941

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (21-91)

Antitoxin

• Uncharacterized protein HI1420 (7-93)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10459.17 Da
• Isoelectric Point: 10.6893

>T169683 WP_179573151.1 NZ_CP060008:3775898-3776191 [Pseudomonas sp. B11D7D]
MAYLIQQTKTFASWHSAVRDLKAKVAIARRIDRAAAGNLGDVKSVGDGVSEMRIDVGAGYRLYFTVRGGIVIVLLVGGDK
STQPADIRQAKKLAKEV

Nucleotide

• Length: 294 bp

>T169683 NZ_CP076655:c29967-29551 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10278.77 Da
• Isoelectric Point: 5.4732

>AT169683 WP_074937747.1 NZ_CP060008:3776194-3776484 [Pseudomonas sp. B11D7D]
MPHTLIPFDMAELLDSEEAVSEYLSQVLADGDTDELIRAIGHVAKARGMSQIAKDSGLGRASLYKALAPGAQPRFDTVIK
VMHALGIELHATPAHT

Nucleotide

• Length: 291 bp

>AT169683 NZ_CP076655:c30194-29964 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7G7RNS1