Detailed information of TA system TA169672

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 39798..40387
• Replicon: plasmid pB2_V3
• Accession: NZ_CP060001
• Organism: Xanthomonas citri pv. citri strain B2

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: M4U123
• Locus tag: HZS92_RS22920
• Protein ID: WP_015463824.1
• Coordinates: 40055..40387 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: M4UHU6
• Locus tag: HZS92_RS22915
• Protein ID: WP_011052949.1
• Coordinates: 39798..40055 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS92_RS22885	36226..36408	+	183	Protein_35	UPF0149 family protein	-
HZS92_RS22890 (HZS92_07042)	36482..37696	+	1215	WP_011050083.1	IS4-like element ISXac1 family transposase	-
HZS92_RS22895 (HZS92_07043)	37715..38284	+	570	Protein_37	UPF0149 family protein	-
HZS92_RS22900 (HZS92_07044)	38305..38535	+	231	WP_268811174.1	Tn3 family transposase	-
HZS92_RS22905 (HZS92_07045)	38702..39006	-	305	Protein_39	Tn3 family transposase	-
HZS92_RS22910 (HZS92_07046)	38990..39592	-	603	WP_011052950.1	recombinase family protein	-
HZS92_RS22915 (HZS92_07047)	39798..40055	+	258	WP_011052949.1	antitoxin	Antitoxin
HZS92_RS22920 (HZS92_07048)	40055..40387	+	333	WP_015463824.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HZS92_RS22925	40410..40661	+	252	WP_125459571.1	hypothetical protein	-
HZS92_RS22930 (HZS92_07049)	40658..40870	+	213	WP_011052947.1	hypothetical protein	-
HZS92_RS22935 (HZS92_07050)	40860..41396	+	537	WP_015463823.1	plasmid pRiA4b ORF-3 family protein	-
HZS92_RS22940	41686..41907	+	222	WP_011052944.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..94653	94653

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11707.46 Da
• Isoelectric Point: 9.1975

>T169672 WP_015463824.1 NZ_CP060001:40055-40387 [Xanthomonas citri pv. citri]
MDRGDVYLVSLDPTSGHEQQGTRPVLIVSPSAFNRLTKTPVVLPITSGGNFARTAGFTVSLMGAGTNTTGVVRCDQPRAL
DLASRRARKLETVPSPIMDEVLAKLSAILE

Nucleotide

• Length: 333 bp

>T169672 NZ_CP076654:1162024-1162335 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9242.70 Da
• Isoelectric Point: 4.7164

>AT169672 WP_011052949.1 NZ_CP060001:39798-40055 [Xanthomonas citri pv. citri]
MHTTNLRKVGGSIMLAVPPAILDMLRLRAGATVGLAVDHGRLVIEPTLRPYYSLDELLAQCDASAELSTEDREWLDAKPV
GSELL

Nucleotide

• Length: 258 bp

>AT169672 NZ_CP076654:1162332-1162751 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: M4U123

Antitoxin

• AlphaFold DB: M4UHU6