Detailed information of TA system TA169671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 99223..99881
• Replicon: plasmid pB2_V1
• Accession: NZ_CP060000
• Organism: Xanthomonas citri pv. citri strain B2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q8PRH3
• Locus tag: HZS92_RS22480
• Protein ID: WP_011053002.1
• Coordinates: 99223..99624 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M4U2L0
• Locus tag: HZS92_RS22485
• Protein ID: WP_011053003.1
• Coordinates: 99621..99881 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS92_RS22460 (HZS92_06126)	94663..95430	-	768	WP_011052998.1	ParA family protein	-
HZS92_RS22465 (HZS92_06128)	96235..97461	-	1227	WP_015472949.1	replication protein RepA	-
HZS92_RS22470 (HZS92_06129)	97741..98928	-	1188	WP_011053001.1	zeta toxin family protein	-
HZS92_RS22475 (HZS92_06130)	98935..99198	-	264	WP_005917846.1	hypothetical protein	-
HZS92_RS22480 (HZS92_06131)	99223..99624	-	402	WP_011053002.1	type II toxin-antitoxin system VapC family toxin	Toxin
HZS92_RS22485 (HZS92_06132)	99621..99881	-	261	WP_011053003.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HZS92_RS22490 (HZS92_06134)	100072..100644	+	573	WP_011053004.1	recombinase family protein	-
HZS92_RS22495 (HZS92_06135)	100675..101263	-	589	Protein_120	IS3-like element ISXac3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..137420	137420

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• SpoVT-AbrB domain (8-46)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14627.05 Da
• Isoelectric Point: 7.2785

>T169671 WP_011053002.1 NZ_CP060000:c99624-99223 [Xanthomonas citri pv. citri]
MKRYMLDTNTVSHLVKSHPAVSRRVIEVPMTALCMSAITGGELMFGLAKVPDAKRLQQAVMELLRRVDVLPWDGAVMERY
GSVRADLEKQGKALGSLDMLIAAHALETDSVLVTNDAAFSRVVGLTVEDWTRS

Nucleotide

• Length: 402 bp

>T169671 NZ_CP076654:1092643-1092978 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9799.00 Da
• Isoelectric Point: 5.0941

>AT169671 WP_011053003.1 NZ_CP060000:c99881-99621 [Xanthomonas citri pv. citri]
VSQVAKLFINGRSQAVRLPAAYRFTEKEVFIRQDPETGDVILSRKPATWEDFFIVLKSADVPANFLDAAERNQGTQDRDP
FAGWRE

Nucleotide

• Length: 261 bp

>AT169671 NZ_CP076654:1092395-1092643 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: Q8PRH3

Antitoxin

• AlphaFold DB: M4U2L0