Detailed information of TA system TA169669

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 59811..60400
• Replicon: plasmid pB2_V1
• Accession: NZ_CP060000
• Organism: Xanthomonas citri pv. citri strain B2

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: M4U123
• Locus tag: HZS92_RS22240
• Protein ID: WP_015463824.1
• Coordinates: 60068..60400 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: M4UHU6
• Locus tag: HZS92_RS22235
• Protein ID: WP_011052949.1
• Coordinates: 59811..60068 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS92_RS22205	56239..56421	+	183	Protein_62	UPF0149 family protein	-
HZS92_RS22210 (HZS92_06073)	56495..57709	+	1215	WP_011050083.1	IS4-like element ISXac1 family transposase	-
HZS92_RS22215 (HZS92_06074)	57728..58297	+	570	Protein_64	UPF0149 family protein	-
HZS92_RS22220 (HZS92_06075)	58318..58548	+	231	WP_268811174.1	Tn3 family transposase	-
HZS92_RS22225 (HZS92_06076)	58715..59019	-	305	Protein_66	Tn3 family transposase	-
HZS92_RS22230 (HZS92_06077)	59003..59605	-	603	WP_011052950.1	recombinase family protein	-
HZS92_RS22235 (HZS92_06078)	59811..60068	+	258	WP_011052949.1	antitoxin	Antitoxin
HZS92_RS22240 (HZS92_06079)	60068..60400	+	333	WP_015463824.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HZS92_RS22245 (HZS92_06080)	60423..60674	+	252	WP_125459571.1	hypothetical protein	-
HZS92_RS22250 (HZS92_06081)	60671..60883	+	213	WP_011052947.1	hypothetical protein	-
HZS92_RS22255 (HZS92_06082)	60873..61409	+	537	WP_015463823.1	plasmid pRiA4b ORF-3 family protein	-
HZS92_RS22260	61699..61920	+	222	WP_011052944.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..137420	137420

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11707.46 Da
• Isoelectric Point: 9.1975

>T169669 WP_015463824.1 NZ_CP060000:60068-60400 [Xanthomonas citri pv. citri]
MDRGDVYLVSLDPTSGHEQQGTRPVLIVSPSAFNRLTKTPVVLPITSGGNFARTAGFTVSLMGAGTNTTGVVRCDQPRAL
DLASRRARKLETVPSPIMDEVLAKLSAILE

Nucleotide

• Length: 333 bp

>T169669 NZ_CP076654:c809518-809141 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9242.70 Da
• Isoelectric Point: 4.7164

>AT169669 WP_011052949.1 NZ_CP060000:59811-60068 [Xanthomonas citri pv. citri]
MHTTNLRKVGGSIMLAVPPAILDMLRLRAGATVGLAVDHGRLVIEPTLRPYYSLDELLAQCDASAELSTEDREWLDAKPV
GSELL

Nucleotide

• Length: 258 bp

>AT169669 NZ_CP076654:c809939-809565 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: M4U123

Antitoxin

• AlphaFold DB: M4UHU6