Detailed information of TA system TA169668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 5769..6358
• Replicon: plasmid pB2_V1
• Accession: NZ_CP060000
• Organism: Xanthomonas citri pv. citri strain B2

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: M4U123
• Locus tag: HZS92_RS21930
• Protein ID: WP_015463824.1
• Coordinates: 5769..6101 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: M4UHU6
• Locus tag: HZS92_RS21935
• Protein ID: WP_011052949.1
• Coordinates: 6101..6358 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS92_RS21910	4249..4470	-	222	WP_011052944.1	hypothetical protein	-
HZS92_RS21915 (HZS92_06007)	4760..5296	-	537	WP_015463823.1	plasmid pRiA4b ORF-3 family protein	-
HZS92_RS21920 (HZS92_06008)	5286..5498	-	213	WP_011052947.1	hypothetical protein	-
HZS92_RS21925 (HZS92_06009)	5495..5746	-	252	WP_125459571.1	hypothetical protein	-
HZS92_RS21930 (HZS92_06010)	5769..6101	-	333	WP_015463824.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HZS92_RS21935 (HZS92_06011)	6101..6358	-	258	WP_011052949.1	antitoxin	Antitoxin
HZS92_RS21940 (HZS92_06012)	6564..7166	+	603	WP_011052950.1	recombinase family protein	-
HZS92_RS21945 (HZS92_06013)	7150..7454	+	305	Protein_9	Tn3 family transposase	-
HZS92_RS21950 (HZS92_06014)	7621..7851	-	231	WP_268811174.1	Tn3 family transposase	-
HZS92_RS21955 (HZS92_06015)	7872..8441	-	570	Protein_11	UPF0149 family protein	-
HZS92_RS21960 (HZS92_06016)	8460..9674	-	1215	WP_011050083.1	IS4-like element ISXac1 family transposase	-
HZS92_RS21965	9748..9930	-	183	Protein_13	UPF0149 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..137420	137420

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11707.46 Da
• Isoelectric Point: 9.1975

>T169668 WP_015463824.1 NZ_CP060000:c6101-5769 [Xanthomonas citri pv. citri]
MDRGDVYLVSLDPTSGHEQQGTRPVLIVSPSAFNRLTKTPVVLPITSGGNFARTAGFTVSLMGAGTNTTGVVRCDQPRAL
DLASRRARKLETVPSPIMDEVLAKLSAILE

Nucleotide

• Length: 333 bp

>T169668 NZ_CP076654:721490-721786 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9242.70 Da
• Isoelectric Point: 4.7164

>AT169668 WP_011052949.1 NZ_CP060000:c6358-6101 [Xanthomonas citri pv. citri]
MHTTNLRKVGGSIMLAVPPAILDMLRLRAGATVGLAVDHGRLVIEPTLRPYYSLDELLAQCDASAELSTEDREWLDAKPV
GSELL

Nucleotide

• Length: 258 bp

>AT169668 NZ_CP076654:721788-722183 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: M4U123

Antitoxin

• AlphaFold DB: M4UHU6