Detailed information of TA system TA169658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5738725..5739311
• Replicon: chromosome
• Accession: NZ_CP059995
• Organism: Pseudomonas aeruginosa strain 401853

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: H5J27_RS27245
• Protein ID: WP_003120987.1
• Coordinates: 5739012..5739311 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: H5J27_RS27240
• Protein ID: WP_003448662.1
• Coordinates: 5738725..5739015 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5J27_RS27225	5734309..5736198	+	1890	WP_023101908.1	hypothetical protein	-
H5J27_RS27230	5736195..5738171	+	1977	WP_031691363.1	DEAD/DEAH box helicase	-
H5J27_RS27235	5738310..5738654	+	345	WP_023082045.1	hypothetical protein	-
H5J27_RS27240	5738725..5739015	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
H5J27_RS27245	5739012..5739311	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H5J27_RS27250	5739513..5740637	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
H5J27_RS27255	5740637..5742346	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
H5J27_RS27260	5742350..5743675	+	1326	WP_126160157.1	type 4b pilus protein PilO2	-
H5J27_RS27265	5743665..5744198	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5707956..5791602	83646

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T169658 WP_003120987.1 NZ_CP059995:c5739311-5739012 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T169658 NZ_CP076651:c2862788-2862585 [Serratia ureilytica]
ATGACTAAAACCGACTATCTGATGCGTTTACGTAAATGCACCACGATTGATACTCTCGAACGTGTTATTGAAAAAAATAA
GTACGAGCTTTCCGATGATGAACTGGAGCTGTTTTACTCAGCCGCCGATCATCGTTTGGCGGAGCTGACCATGAACAAGC
TGTATGACAAAATCCCCACCTCCGTTTGGAAATATGTCAGATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT169658 WP_003448662.1 NZ_CP059995:c5739015-5738725 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT169658 NZ_CP076651:c2863207-2862839 [Serratia ureilytica]
ATGGATGAGTATTCGCCTAAGCGACACGACATTGCTCAACTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGTATCGC
CACCCTGGGAGACAGCCACCACGGGTGGGTCAACGATCCGACATCCTCCGTCAACCTTCAGCTTAATGAGTTGATCGAAC
ATATCGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAATCCGATTTGTCGGAGTTAGTCGAGGAATATCTC
GACGATACCTATACGCTGTTCAGCAGCTACGGTATCAATGATTCCGACCTGCGCCGCTGGCAAAAAACCAAGGCGAGATT
ATTCAGAATGTTCTCAGGAGAGGACATCTGTACGACAATGAAAACTTAG

Structures

Toxin

• AlphaFold DB: G8CP73