Detailed information of TA system TA169656

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 5269311..5269919
• Replicon: chromosome
• Accession: NZ_CP059995
• Organism: Pseudomonas aeruginosa strain 401853

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: H5J27_RS24970
• Protein ID: WP_042168819.1
• Coordinates: 5269572..5269919 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0B0C355
• Locus tag: H5J27_RS24965
• Protein ID: WP_003114155.1
• Coordinates: 5269311..5269562 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5J27_RS24945	5264984..5265508	+	525	WP_042168829.1	helix-turn-helix domain-containing protein	-
H5J27_RS24950	5265505..5266773	+	1269	WP_042168826.1	HipA domain-containing protein	-
H5J27_RS24955	5266848..5267879	-	1032	WP_042168823.1	fimbrial protein	-
H5J27_RS24960	5268507..5269097	+	591	WP_079387396.1	tyrosine-type recombinase/integrase	-
H5J27_RS24965	5269311..5269562	+	252	WP_003114155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
H5J27_RS24970	5269572..5269919	+	348	WP_042168819.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H5J27_RS24975	5270196..5270837	-	642	WP_003158902.1	substrate-binding domain-containing protein	-
H5J27_RS24980	5270959..5271264	+	306	WP_003158903.1	helix-turn-helix domain-containing protein	-
H5J27_RS24985	5271363..5273873	-	2511	WP_003160813.1	S8 family peptidase	-
H5J27_RS24990	5273851..5274882	-	1032	WP_003158906.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	sul1 / blaOXA-10 / blaIMP-14 / catB7	-	5155619..5374983	219364

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12978.82 Da
• Isoelectric Point: 4.5511

>T169656 WP_042168819.1 NZ_CP059995:5269572-5269919 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGELAALQSVLSLLDEIEEKISLAPKGYPVSHQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Nucleotide

• Length: 348 bp

>T169656 NZ_CP076651:c1797151-1796771 [Serratia ureilytica]
ATGGTGGCTCAACGGGCGTTATTCGACACCAATATCCTGATCGATTATCTCAACGGCATTCCTCAAGCCAGGGACGTTCT
GGTCGAGTATCACATCAATCCGGCCATCAGCGCCATCACCTGGATGGAAGTGATGGTAGGTGCTAAAAAACAGGGTCCGG
CACTGGAGCTGAAAACACGTCAGTTTTTAGGGCAATTTCTGCTGCTGCCTATTACCGATGAAGTCGCCGAACGCGCCGTA
GAACTGCGCCACTCACAGCATGTGAAACTGCCAGACGCCATTATCTGGGCCACCGCGCAGGTGGGATTCCGGATGCTCAT
CTCCCGCAACCCCAAAGATTTCGGCACTGACAATGGCGTGTTGATGCCTTATCGGCTGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9442.79 Da
• Isoelectric Point: 9.4808

>AT169656 WP_003114155.1 NZ_CP059995:5269311-5269562 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT169656 NZ_CP076651:c1797372-1797151 [Serratia ureilytica]
ATGACTCGCATCGTGATCGATTTACCTGAAGAAGACCTGCGGCAGCTCGATAATCTGAAAAATATCCGCCACGTTCCCCG
GGCCGAAATCATTCGTCAAGCAGTCTCCCGCTATCTGACGGAGAACCACGTGGAAGATCCCAGCAACGCTTTCGGGCTCT
GGTCCGGGGCGATGGGAGATGGCGTGGAGTACGAAAATCGTCAGCGCGAGGAGTGGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0B0C355