Detailed information of TA system TA169650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 55849..56507
• Replicon: plasmid pT4p1
• Accession: NZ_CP059993
• Organism: Xanthomonas citri strain T4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q8PRH3
• Locus tag: HZS93_RS22515
• Protein ID: WP_011053002.1
• Coordinates: 56106..56507 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M4U2L0
• Locus tag: HZS93_RS22510
• Protein ID: WP_011053003.1
• Coordinates: 55849..56109 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS93_RS22500 (HZS93_06071)	54467..55055	+	589	Protein_59	IS3-like element ISXac3 family transposase	-
HZS93_RS22505 (HZS93_06072)	55086..55658	-	573	WP_011053004.1	recombinase family protein	-
HZS93_RS22510 (HZS93_06074)	55849..56109	+	261	WP_011053003.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HZS93_RS22515 (HZS93_06075)	56106..56507	+	402	WP_011053002.1	type II toxin-antitoxin system VapC family toxin	Toxin
HZS93_RS22520 (HZS93_06076)	56532..56795	+	264	WP_005917846.1	hypothetical protein	-
HZS93_RS22525 (HZS93_06077)	56802..57989	+	1188	WP_011053001.1	zeta toxin family protein	-
HZS93_RS22530 (HZS93_06078)	58269..59495	+	1227	WP_015472949.1	replication protein RepA	-
HZS93_RS22535 (HZS93_06080)	60300..61067	+	768	WP_011052998.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..99130	99130

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• SpoVT-AbrB domain (8-46)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14627.05 Da
• Isoelectric Point: 7.2785

>T169650 WP_011053002.1 NZ_CP059993:56106-56507 [Xanthomonas citri]
MKRYMLDTNTVSHLVKSHPAVSRRVIEVPMTALCMSAITGGELMFGLAKVPDAKRLQQAVMELLRRVDVLPWDGAVMERY
GSVRADLEKQGKALGSLDMLIAAHALETDSVLVTNDAAFSRVVGLTVEDWTRS

Nucleotide

• Length: 402 bp

>T169650 NZ_CP076650:30908-31189 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9799.00 Da
• Isoelectric Point: 5.0941

>AT169650 WP_011053003.1 NZ_CP059993:55849-56109 [Xanthomonas citri]
VSQVAKLFINGRSQAVRLPAAYRFTEKEVFIRQDPETGDVILSRKPATWEDFFIVLKSADVPANFLDAAERNQGTQDRDP
FAGWRE

Nucleotide

• Length: 261 bp

>AT169650 NZ_CP076650:30667-30918 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: Q8PRH3

Antitoxin

• AlphaFold DB: M4U2L0