Detailed information of TA system TA169649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 16292..16881
• Replicon: plasmid pT4p1
• Accession: NZ_CP059993
• Organism: Xanthomonas citri strain T4

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: M4U123
• Locus tag: HZS93_RS22325
• Protein ID: WP_015463824.1
• Coordinates: 16292..16624 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: M4UHU6
• Locus tag: HZS93_RS22330
• Protein ID: WP_011052949.1
• Coordinates: 16624..16881 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS93_RS22305	14772..14993	-	222	WP_011052944.1	hypothetical protein	-
HZS93_RS22310 (HZS93_06024)	15283..15819	-	537	WP_015463823.1	plasmid pRiA4b ORF-3 family protein	-
HZS93_RS22315 (HZS93_06025)	15809..16021	-	213	WP_011052947.1	hypothetical protein	-
HZS93_RS22320	16018..16269	-	252	WP_125459571.1	hypothetical protein	-
HZS93_RS22325 (HZS93_06026)	16292..16624	-	333	WP_015463824.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HZS93_RS22330 (HZS93_06027)	16624..16881	-	258	WP_011052949.1	antitoxin	Antitoxin
HZS93_RS22335 (HZS93_06028)	17087..17689	+	603	WP_011052950.1	recombinase family protein	-
HZS93_RS22340 (HZS93_06029)	17673..17977	+	305	Protein_27	Tn3 family transposase	-
HZS93_RS22345 (HZS93_06030)	18144..18302	-	159	WP_011052952.1	Tn3 family transposase	-
HZS93_RS22350 (HZS93_06031)	18395..18964	-	570	Protein_29	UPF0149 family protein	-
HZS93_RS22355 (HZS93_06032)	18983..20197	-	1215	WP_011050083.1	IS4-like element ISXac1 family transposase	-
HZS93_RS22360	20313..20453	-	141	Protein_31	UPF0149 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..99130	99130

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11707.46 Da
• Isoelectric Point: 9.1975

>T169649 WP_015463824.1 NZ_CP059993:c16624-16292 [Xanthomonas citri]
MDRGDVYLVSLDPTSGHEQQGTRPVLIVSPSAFNRLTKTPVVLPITSGGNFARTAGFTVSLMGAGTNTTGVVRCDQPRAL
DLASRRARKLETVPSPIMDEVLAKLSAILE

Nucleotide

• Length: 333 bp

>T169649 NZ_CP076649:76791-77207 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9242.70 Da
• Isoelectric Point: 4.7164

>AT169649 WP_011052949.1 NZ_CP059993:c16881-16624 [Xanthomonas citri]
MHTTNLRKVGGSIMLAVPPAILDMLRLRAGATVGLAVDHGRLVIEPTLRPYYSLDELLAQCDASAELSTEDREWLDAKPV
GSELL

Nucleotide

• Length: 258 bp

>AT169649 NZ_CP076649:76564-76794 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: M4U123

Antitoxin

• AlphaFold DB: M4UHU6