Detailed information of TA system TA169645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 2579497..2580130
• Replicon: chromosome
• Accession: NZ_CP059992
• Organism: Xanthomonas citri strain T4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HZS93_RS11265
• Protein ID: WP_015463212.1
• Coordinates: 2579497..2579910 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M4TWF0
• Locus tag: HZS93_RS11270
• Protein ID: WP_015463213.1
• Coordinates: 2579897..2580130 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS93_RS11250 (HZS93_02368)	2575435..2577369	+	1935	WP_230598900.1	TonB-dependent siderophore receptor	-
HZS93_RS11255 (HZS93_02369)	2577578..2579092	+	1515	WP_015463210.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
HZS93_RS11260 (HZS93_02370)	2579108..2579470	-	363	WP_015463211.1	DUF3742 family protein	-
HZS93_RS11265 (HZS93_02371)	2579497..2579910	-	414	WP_015463212.1	PIN domain-containing protein	Toxin
HZS93_RS11270 (HZS93_02372)	2579897..2580130	-	234	WP_015463213.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HZS93_RS11275 (HZS93_02373)	2580412..2581419	+	1008	WP_015463214.1	DUF4917 family protein	-
HZS93_RS11280 (HZS93_02374)	2581552..2582007	-	456	WP_015463215.1	hypothetical protein	-
HZS93_RS11285 (HZS93_02375)	2582402..2582977	+	576	WP_011051400.1	sigma-70 family RNA polymerase sigma factor	-
HZS93_RS11290 (HZS93_02376)	2582958..2584007	+	1050	WP_011051401.1	FecR domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-119)

Antitoxin

• SpoVT-AbrB domain (5-45)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15221.65 Da
• Isoelectric Point: 6.3225

>T169645 WP_015463212.1 NZ_CP059992:c2579910-2579497 [Xanthomonas citri]
MAAGKGKVFLDSNVVLYLLSEDAVKADGAEALLQRRPVISVQVLNEVTHVCVRKLKMGWDEVGQFLALVRSFCKIVPLTV
DVHDRARQLAERHQLSFYDACIVAAAAIEGCQTLYSEDMHHGLIIEESLSIRNPFNV

Nucleotide

• Length: 414 bp

>T169645 NZ_CP076646:c4219178-4219005 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8663.97 Da
• Isoelectric Point: 8.7848

>AT169645 WP_015463213.1 NZ_CP059992:c2580130-2579897 [Xanthomonas citri]
MQVAKWGNSLAVRLPSSLVEALELREGDDIEIVVDDPRLFAVRKKPGPEAMLERLRAFRGKLPADFKFNRDEANGRG

Nucleotide

• Length: 234 bp

>AT169645 NZ_CP076646:c4219502-4219206 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: M4TWF0