Detailed information of TA system TA169643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 3468632..3469197
• Replicon: chromosome
• Accession: NZ_CP059991
• Organism: Streptomyces gardneri strain ATCC 15439

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: H4W23_RS15575
• Protein ID: WP_055643226.1
• Coordinates: 3468632..3469003 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A5P2B477
• Locus tag: H4W23_RS15580
• Protein ID: WP_055646260.1
• Coordinates: 3469000..3469197 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4W23_RS15560	3464649..3465629	+	981	WP_055643228.1	winged helix-turn-helix domain-containing protein	-
H4W23_RS15565	3465637..3466278	-	642	WP_017237054.1	helix-turn-helix domain-containing protein	-
H4W23_RS15570	3466718..3468508	-	1791	WP_079037582.1	DEAD/DEAH box helicase	-
H4W23_RS15575	3468632..3469003	-	372	WP_055643226.1	Fic family protein	Toxin
H4W23_RS15580	3469000..3469197	-	198	WP_055646260.1	Arc family DNA-binding protein	Antitoxin
H4W23_RS15585	3469231..3470727	-	1497	WP_055643225.1	MFS transporter	-
H4W23_RS15590	3470910..3471695	-	786	WP_055643224.1	ABC transporter permease	-
H4W23_RS15595	3471802..3472779	-	978	WP_055643223.1	ATP-binding cassette domain-containing protein	-
H4W23_RS15600	3472835..3473506	-	672	WP_055643222.1	DUF4097 family beta strand repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (16-120)

Antitoxin

• Arc-like DNA binding domain (5-43)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13701.71 Da
• Isoelectric Point: 4.8500

>T169643 WP_055643226.1 NZ_CP059991:c3469003-3468632 [Streptomyces gardneri]
VTYFLTLPELLNLAGRLGASEVRDYGLLESALARPQTSVFGQDAYPEIWEKAAALMESLARNHGLVDGNKRIAWYATWVF
LHMNGHPLDPAFDVDEAEMFVLDVCQGALDVPKIAARLPRFAR

Nucleotide

• Length: 372 bp

>T169643 NZ_CP076646:c3741413-3741015 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7334.23 Da
• Isoelectric Point: 6.2200

>AT169643 WP_055646260.1 NZ_CP059991:c3469197-3469000 [Streptomyces gardneri]
MAGLNVRFTQEELDALRERAEAEGRSMQSFAHEAVIKAINEHSRLFNEAAEHVLKASAELNRRLA

Nucleotide

• Length: 198 bp

>AT169643 NZ_CP076646:c3741709-3741416 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A5P2B477