Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4579799..4580550 Replicon chromosome
Accession NZ_CP059963
Organism Pectobacterium brasiliense strain IPO:3649 NAK:375

Toxin (Protein)


Gene name ataT Uniprot ID A0A433N1V3
Locus tag H5A23_RS20200 Protein ID WP_010281603.1
Coordinates 4579799..4580281 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A0M2EWB4
Locus tag H5A23_RS20205 Protein ID WP_039317395.1
Coordinates 4580272..4580550 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5A23_RS20180 4575402..4576475 - 1074 WP_119870807.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -
H5A23_RS20185 4576501..4577346 - 846 WP_010281598.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
H5A23_RS20190 4577372..4578259 - 888 WP_010281599.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
H5A23_RS20195 4578332..4579666 - 1335 WP_119870806.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
H5A23_RS20200 4579799..4580281 - 483 WP_010281603.1 GNAT family N-acetyltransferase Toxin
H5A23_RS20205 4580272..4580550 - 279 WP_039317395.1 DUF1778 domain-containing protein Antitoxin
H5A23_RS20210 4580660..4581076 - 417 WP_119870805.1 hypothetical protein -
H5A23_RS20215 4581436..4581915 + 480 WP_039511462.1 Hcp family type VI secretion system effector -
H5A23_RS20220 4581917..4582765 + 849 WP_119870804.1 DUF4225 domain-containing protein -
H5A23_RS20225 4582762..4583160 - 399 WP_127110965.1 hypothetical protein -
H5A23_RS20230 4583381..4584139 - 759 WP_119870824.1 DeoR/GlpR family DNA-binding transcription regulator -
H5A23_RS20235 4584477..4585391 + 915 WP_119870802.1 NAD(P)-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(39-144)

Antitoxin

Vibrio phage ICP1, Orf50

(2-92)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17509.30 Da        Isoelectric Point: 9.0439

>T169634 WP_010281603.1 NZ_CP059963:c4580281-4579799 [Pectobacterium brasiliense]
MGITAPEPLSSSHNLAEFCSQEPSLNEWLKKKALKNHSAGISRVYVICAEGTHQVIGYYCLSTGSVQRNTAPGAYRRNAP
ESLPVIVLGRLAIDQAYSGKGLGVALLKDAIFRTENIALQVGVRALVVHALDENVRNFYLKFAFDPSPVQPLMLLYPIKI

Download         Length: 483 bp

>T169634 NZ_CP076646:1825540-1825791 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10667.12 Da        Isoelectric Point: 4.3611

>AT169634 WP_039317395.1 NZ_CP059963:c4580550-4580272 [Pectobacterium brasiliense]
MKPETKEAPINIRAKASQRDLIDMAANLVSKSRTDFMLEAACREAQDILLDQRLFILDNEQFDEFLAELDAPITEERQAR
INDLMSRKSPWE

Download         Length: 279 bp

>AT169634 NZ_CP076646:1825788-1826042 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A433N1V3

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2EWB4

References