Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 4542054..4542671 Replicon chromosome
Accession NZ_CP059963
Organism Pectobacterium brasiliense strain IPO:3649 NAK:375

Toxin (Protein)


Gene name hicA Uniprot ID C6DHN3
Locus tag H5A23_RS20000 Protein ID WP_010281507.1
Coordinates 4542054..4542233 (+) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A086ESS8
Locus tag H5A23_RS20005 Protein ID WP_014916995.1
Coordinates 4542261..4542671 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5A23_RS19975 4537295..4538455 - 1161 WP_039465321.1 cystathionine gamma-synthase -
H5A23_RS19980 4538643..4538960 + 318 WP_005973355.1 met regulon transcriptional regulator MetJ -
H5A23_RS19985 4539194..4539991 + 798 WP_119870818.1 ABC transporter ATP-binding protein -
H5A23_RS19990 4540001..4540963 + 963 WP_014916993.1 ABC transporter substrate-binding protein -
H5A23_RS19995 4540960..4541970 + 1011 WP_119870817.1 iron ABC transporter permease -
H5A23_RS20000 4542054..4542233 + 180 WP_010281507.1 type II toxin-antitoxin system HicA family toxin Toxin
H5A23_RS20005 4542261..4542671 + 411 WP_014916995.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H5A23_RS20010 4542768..4542983 - 216 WP_010281510.1 50S ribosomal protein L31 -
H5A23_RS20015 4543257..4545455 + 2199 WP_104209601.1 primosomal protein N' -
H5A23_RS20020 4545764..4546807 + 1044 WP_010281514.1 DNA-binding transcriptional regulator CytR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6560.69 Da        Isoelectric Point: 10.7838

>T169632 WP_010281507.1 NZ_CP059963:4542054-4542233 [Pectobacterium brasiliense]
MDSRTLIAEIKADGWELIRVNGSHHHFMHPSKPGLVTIPHPKKDLPIGTVKSIRKQAGI

Download         Length: 180 bp

>T169632 NZ_CP076646:882446-882853 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 14506.39 Da        Isoelectric Point: 4.3391

>AT169632 WP_014916995.1 NZ_CP059963:4542261-4542671 [Pectobacterium brasiliense]
MFYPIAIEAGDDTHAYGVTVPDLPGCFSAGDTLDDAIANAKEAITGHIELLIEMGQDIPTVSSVGQLAKSAEYAGYTWAV
VDIDVTRLMGGSEKINVTLPKSLIDRIDRCVASNPEFKSRSGFLAQAALERISSSR

Download         Length: 411 bp

>AT169632 NZ_CP076646:882199-882465 [Escherichia coli]
ATGGATATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W2I1S0

Antitoxin

Source ID Structure
AlphaFold DB A0A086ESS8

References