Detailed information of TA system TA169629

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1746107..1746660
• Replicon: chromosome
• Accession: NZ_CP059963
• Organism: Pectobacterium brasiliense strain IPO:3649 NAK:375

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: H5A23_RS07625
• Protein ID: WP_119870392.1
• Coordinates: 1746346..1746660 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A3S1FM98
• Locus tag: H5A23_RS07620
• Protein ID: WP_029729596.1
• Coordinates: 1746107..1746343 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A23_RS07610	1741769..1744078	-	2310	WP_119870906.1	TerB N-terminal domain-containing protein	-
H5A23_RS07615	1744843..1745787	+	945	WP_119870391.1	DUF1738 domain-containing protein	-
H5A23_RS07620	1746107..1746343	+	237	WP_029729596.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
H5A23_RS07625	1746346..1746660	+	315	WP_119870392.1	CcdB family protein	Toxin
H5A23_RS07630	1747084..1748082	+	999	WP_119870393.1	hypothetical protein	-
H5A23_RS07635	1748245..1748787	-	543	WP_119870394.1	DUF3916 domain-containing protein	-
H5A23_RS07640	1748984..1749730	-	747	WP_119870395.1	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
H5A23_RS07645	1749739..1751601	-	1863	WP_119870396.1	type IV secretory system conjugative DNA transfer family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1731522..1771717	40195

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11569.51 Da
• Isoelectric Point: 7.9859

>T169629 WP_119870392.1 NZ_CP059963:1746346-1746660 [Pectobacterium brasiliense]
MQFTVYGNTGKSVVYPLLLDVTSDIIGQLNRRIMIPLLPIEKYPAGRRPDRLVPVVRLTDGKEYAVMTHELASIPVQALG
AVFCDASQYRSQVKASIDFLIDGF

Nucleotide

• Length: 315 bp

>T169629 NZ_CP076646:620205-620519 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGAG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8927.96 Da
• Isoelectric Point: 6.4612

>AT169629 WP_029729596.1 NZ_CP059963:1746107-1746343 [Pectobacterium brasiliense]
MTAKQRNTQSVTMTVERALLVRAREAGINLSATLTTALDAELRSHEAKKWQEENREALAALNRFHDEQGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT169629 NZ_CP076646:620516-620932 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A3S1FM98