Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1214679..1215304 Replicon chromosome
Accession NZ_CP059963
Organism Pectobacterium brasiliense strain IPO:3649 NAK:375

Toxin (Protein)


Gene name Hha Uniprot ID C6DB72
Locus tag H5A23_RS05530 Protein ID WP_005976087.1
Coordinates 1214679..1214882 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q6D809
Locus tag H5A23_RS05535 Protein ID WP_010282247.1
Coordinates 1214936..1215304 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5A23_RS05500 1210012..1210350 + 339 WP_002208627.1 P-II family nitrogen regulator -
H5A23_RS05505 1210390..1211676 + 1287 WP_185893748.1 ammonium transporter AmtB -
H5A23_RS05510 1211816..1212679 - 864 WP_119872106.1 acyl-CoA thioesterase II -
H5A23_RS05515 1212892..1213473 + 582 WP_119872105.1 YbaY family lipoprotein -
H5A23_RS05520 1213475..1213813 - 339 WP_119872104.1 MGMT family protein -
H5A23_RS05530 1214679..1214882 - 204 WP_005976087.1 hemolysin expression modulator Hha Toxin
H5A23_RS05535 1214936..1215304 - 369 WP_010282247.1 Hha toxicity modulator TomB Antitoxin
H5A23_RS05540 1215903..1216046 - 144 WP_005976091.1 type B 50S ribosomal protein L36 -
H5A23_RS05545 1216064..1216312 - 249 WP_039274617.1 type B 50S ribosomal protein L31 -
H5A23_RS05550 1216543..1219671 - 3129 WP_039511040.1 multidrug efflux RND transporter permease subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8145.52 Da        Isoelectric Point: 8.9008

>T169628 WP_005976087.1 NZ_CP059963:c1214882-1214679 [Pectobacterium brasiliense]
MKKIDYLMRLRKCTTIDTLERVIEKNKYELSNDELEMFFSAADHRLAELTMNKLYDKVPTAVWRYVR

Download         Length: 204 bp

>T169628 NZ_CP076646:180802-180909 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14046.80 Da        Isoelectric Point: 4.4787

>AT169628 WP_010282247.1 NZ_CP059963:c1215304-1214936 [Pectobacterium brasiliense]
MDEYTPKHYDIAQLRFLCENLCDESIATLGDSSHGWVNDPTSAINLQLNELIEHIATFILTFKIKYPNESELSEQVEKYL
DDTYVLFSNYGINDAELRRWQKSKAKLFGMFSGENICTPAKT

Download         Length: 369 bp

>AT169628 NZ_CP076646:c180754-180688 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D7Z3F0

Antitoxin

Source ID Structure
AlphaFold DB A0A086EI31

References