Detailed information of TA system TA169626

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 540858..541518
• Replicon: chromosome
• Accession: NZ_CP059963
• Organism: Pectobacterium brasiliense strain IPO:3649 NAK:375

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: H5A23_RS02575
• Protein ID: WP_072061666.1
• Coordinates: 541105..541518 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A1D7Z1Z6
• Locus tag: H5A23_RS02570
• Protein ID: WP_005971623.1
• Coordinates: 540858..541124 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A23_RS02550	536821..537825	-	1005	WP_119870587.1	LacI family DNA-binding transcriptional regulator	-
H5A23_RS02555	537880..538488	-	609	WP_010279828.1	HD domain-containing protein	-
H5A23_RS02560	538919..539566	+	648	WP_010279833.1	hemolysin III family protein	-
H5A23_RS02565	539621..540622	-	1002	WP_010279836.1	tRNA-modifying protein YgfZ	-
H5A23_RS02570	540858..541124	+	267	WP_005971623.1	FAD assembly factor SdhE	Antitoxin
H5A23_RS02575	541105..541518	+	414	WP_072061666.1	protein YgfX	Toxin
H5A23_RS02580	541587..542522	+	936	WP_119870588.1	23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF	-
H5A23_RS02585	542588..542911	-	324	WP_119870589.1	hypothetical protein	-
H5A23_RS02590	542940..543458	-	519	WP_119870590.1	flavodoxin FldB	-
H5A23_RS02595	543801..544187	-	387	WP_048256533.1	acyl-CoA thioesterase	-
H5A23_RS02600	544392..545720	-	1329	WP_039282120.1	MHS family MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-132)

Antitoxin

• Flavinator of succinate dehydrogenase (8-79)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 16325.29 Da
• Isoelectric Point: 10.7911

>T169626 WP_072061666.1 NZ_CP059963:541105-541518 [Pectobacterium brasiliense]
VAQWRCDLRVSWRMQLLSLVVHGLLVLLILLAPWPDGYAWLWLCLVTMVMFGFIRSQRNIKSRQGEIVLLSETTLNWRQQ
EWQIVKRPWLLKNGILLSLQAVNGKDKQQLWLASDSMGDDEWRHLRQILLQQKHWAR

Nucleotide

• Length: 414 bp

>T169626 NZ_CP076645:4541612-4541989 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCACCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCGACTGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10507.94 Da
• Isoelectric Point: 5.1392

>AT169626 WP_005971623.1 NZ_CP059963:540858-541124 [Pectobacterium brasiliense]
MEIDNKSRIHWACRRGMRELDIAIMPFFEHDYDTLNDTDKRTFVRLLQSDDPDLFNWLMNHGEPEDGELKRMISLIQTRN
KDRGPVAM

Nucleotide

• Length: 267 bp

>AT169626 NZ_CP076645:4541191-4541565 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCACCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCACCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGACTGTTTATCCGACACCGGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A1D7Z1Z6