Detailed information of TA system TA169625

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 358712..359238
• Replicon: chromosome
• Accession: NZ_CP059963
• Organism: Pectobacterium brasiliense strain IPO:3649 NAK:375

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: H5A23_RS01635
• Protein ID: WP_119871147.1
• Coordinates: 358712..359017 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: C6C380
• Locus tag: H5A23_RS01640
• Protein ID: WP_015855076.1
• Coordinates: 359020..359238 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A23_RS01605	353713..353967	-	255	WP_119871224.1	helix-turn-helix domain-containing protein	-
H5A23_RS01610	354071..354313	+	243	WP_072010299.1	helix-turn-helix domain-containing protein	-
H5A23_RS01615	354653..354946	+	294	WP_038921611.1	DUF1778 domain-containing protein	-
H5A23_RS01620	354943..355437	+	495	WP_119871144.1	GNAT family N-acetyltransferase	-
H5A23_RS01625	355863..357584	+	1722	WP_119871145.1	AAA family ATPase	-
H5A23_RS01630	357670..358512	+	843	WP_119871146.1	DNA adenine methylase	-
H5A23_RS01635	358712..359017	-	306	WP_119871147.1	type II toxin-antitoxin system toxin CcdB	Toxin
H5A23_RS01640	359020..359238	-	219	WP_015855076.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
H5A23_RS01645	359297..360040	-	744	WP_119871148.1	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
H5A23_RS01650	360133..360483	+	351	WP_158586005.1	hypothetical protein	-
H5A23_RS01655	360937..361278	-	342	WP_119871225.1	hypothetical protein	-
H5A23_RS01660	361464..361658	+	195	WP_044209559.1	DUF3950 domain-containing protein	-
H5A23_RS01665	361774..363036	-	1263	WP_119871150.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	354397..363213	8816

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11759.63 Da
• Isoelectric Point: 4.6533

>T169625 WP_119871147.1 NZ_CP059963:c359017-358712 [Pectobacterium brasiliense]
MQFIVYQYKRASHYKMFVDVQSDIVETPKRRMVIPLIEAYHLSEKVNKTLFPLIRIDGEDYRLMTTELSSVPVEVIGEVT
ADLGDYADEIKDAINLMFWGI

Nucleotide

• Length: 306 bp

>T169625 NZ_CP076645:3962526-3962804 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACTGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8178.13 Da
• Isoelectric Point: 7.0135

>AT169625 WP_015855076.1 NZ_CP059963:c359238-359020 [Pectobacterium brasiliense]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFIAQNGSFADENRNW

Nucleotide

• Length: 219 bp

>AT169625 NZ_CP076645:3962263-3962523 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACTATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGGTGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A250BG46