Detailed information of TA system TA169624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 354653..355437
• Replicon: chromosome
• Accession: NZ_CP059963
• Organism: Pectobacterium brasiliense strain IPO:3649 NAK:375

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: H5A23_RS01620
• Protein ID: WP_119871144.1
• Coordinates: 354943..355437 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A3S0Y1Y3
• Locus tag: H5A23_RS01615
• Protein ID: WP_038921611.1
• Coordinates: 354653..354946 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A23_RS01590	349960..350745	+	786	WP_185893700.1	phosphonate metabolism protein PhnP	-
H5A23_RS01595	350973..352727	+	1755	WP_119871142.1	AIPR family protein	-
H5A23_RS01600	353380..353628	-	249	WP_119871143.1	helix-turn-helix domain-containing protein	-
H5A23_RS01605	353713..353967	-	255	WP_119871224.1	helix-turn-helix domain-containing protein	-
H5A23_RS01610	354071..354313	+	243	WP_072010299.1	helix-turn-helix domain-containing protein	-
H5A23_RS01615	354653..354946	+	294	WP_038921611.1	DUF1778 domain-containing protein	Antitoxin
H5A23_RS01620	354943..355437	+	495	WP_119871144.1	GNAT family N-acetyltransferase	Toxin
H5A23_RS01625	355863..357584	+	1722	WP_119871145.1	AAA family ATPase	-
H5A23_RS01630	357670..358512	+	843	WP_119871146.1	DNA adenine methylase	-
H5A23_RS01635	358712..359017	-	306	WP_119871147.1	type II toxin-antitoxin system toxin CcdB	-
H5A23_RS01640	359020..359238	-	219	WP_015855076.1	type II toxin-antitoxin system antitoxin CcdA	-
H5A23_RS01645	359297..360040	-	744	WP_119871148.1	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	354397..363213	8816

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17689.53 Da
• Isoelectric Point: 7.2150

>T169624 WP_119871144.1 NZ_CP059963:354943-355437 [Pectobacterium brasiliense]
MISAPEPLRAEHILSSFCCGVESMDNWLKQRAMKNQVTGASRTFVSCDDSKVLAYYSLASSAVATNAAPGRFRRNMPDPI
PVVVLGRLAVDTSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEAREFYLRVGFEPSPIDPMMLMVTLGDLVG
SLSI

Nucleotide

• Length: 495 bp

>T169624 NZ_CP076645:c3956571-3956173 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10856.44 Da
• Isoelectric Point: 10.1968

>AT169624 WP_038921611.1 NZ_CP059963:354653-354946 [Pectobacterium brasiliense]
MPATNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT169624 NZ_CP076645:c3956867-3956574 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A3S0Y1Y3