169620

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5034084..5034610 Replicon chromosome
Accession NZ_CP059962
Organism Pectobacterium brasiliense strain IPO:3710 NAK:380

Toxin (Protein)


Gene name ccdB Uniprot ID -
Locus tag H5A25_RS22185 Protein ID WP_119871147.1
Coordinates 5034084..5034389 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID C6C380
Locus tag H5A25_RS22190 Protein ID WP_015855076.1
Coordinates 5034392..5034610 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5A25_RS22155 5029085..5029339 - 255 WP_119871224.1 helix-turn-helix domain-containing protein -
H5A25_RS22160 5029443..5029685 + 243 WP_072010299.1 helix-turn-helix domain-containing protein -
H5A25_RS22165 5030025..5030318 + 294 WP_038921611.1 DUF1778 domain-containing protein -
H5A25_RS22170 5030315..5030809 + 495 WP_119871144.1 GNAT family N-acetyltransferase -
H5A25_RS22175 5031235..5032956 + 1722 WP_119871145.1 AAA family ATPase -
H5A25_RS22180 5033042..5033884 + 843 WP_119871146.1 DNA adenine methylase -
H5A25_RS22185 5034084..5034389 - 306 WP_119871147.1 type II toxin-antitoxin system toxin CcdB Toxin
H5A25_RS22190 5034392..5034610 - 219 WP_015855076.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H5A25_RS22195 5034669..5035412 - 744 WP_119871148.1 molybdopterin-guanine dinucleotide biosynthesis protein MobC -
H5A25_RS22200 5035505..5035855 + 351 WP_158586005.1 hypothetical protein -
H5A25_RS22205 5036309..5036650 - 342 WP_119871225.1 hypothetical protein -
H5A25_RS22210 5036836..5037030 + 195 WP_044209559.1 DUF3950 domain-containing protein -
H5A25_RS22215 5037146..5038408 - 1263 WP_119871150.1 integrase arm-type DNA-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 5029769..5038585 8816


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11759.63 Da        Isoelectric Point: 4.6533

>T169620 WP_119871147.1 NZ_CP059962:c5034389-5034084 [Pectobacterium brasiliense]
MQFIVYQYKRASHYKMFVDVQSDIVETPKRRMVIPLIEAYHLSEKVNKTLFPLIRIDGEDYRLMTTELSSVPVEVIGEVT
ADLGDYADEIKDAINLMFWGI

Download         Length: 306 bp

>T169620 NZ_CP076645:2827864-2827971 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8178.13 Da        Isoelectric Point: 7.0135

>AT169620 WP_015855076.1 NZ_CP059962:c5034610-5034392 [Pectobacterium brasiliense]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFIAQNGSFADENRNW

Download         Length: 219 bp

>AT169620 NZ_CP076645:c2827817-2827751 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A250BG46

References