Detailed information of TA system TA169611

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 4202837..4203588
• Replicon: chromosome
• Accession: NZ_CP059962
• Organism: Pectobacterium brasiliense strain IPO:3710 NAK:380

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A433N1V3
• Locus tag: H5A25_RS18445
• Protein ID: WP_010281603.1
• Coordinates: 4202837..4203319 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A0M2EWB4
• Locus tag: H5A25_RS18450
• Protein ID: WP_039317395.1
• Coordinates: 4203310..4203588 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A25_RS18425	4198440..4199513	-	1074	WP_119870807.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-
H5A25_RS18430	4199539..4200384	-	846	WP_010281598.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
H5A25_RS18435	4200410..4201297	-	888	WP_010281599.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
H5A25_RS18440	4201370..4202704	-	1335	WP_119870806.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
H5A25_RS18445	4202837..4203319	-	483	WP_010281603.1	GNAT family N-acetyltransferase	Toxin
H5A25_RS18450	4203310..4203588	-	279	WP_039317395.1	DUF1778 domain-containing protein	Antitoxin
H5A25_RS18455	4203698..4204114	-	417	WP_119870805.1	hypothetical protein	-
H5A25_RS18460	4204474..4204953	+	480	WP_039511462.1	Hcp family type VI secretion system effector	-
H5A25_RS18465	4204955..4205803	+	849	WP_119870804.1	DUF4225 domain-containing protein	-
H5A25_RS18470	4205800..4206198	-	399	WP_127110965.1	hypothetical protein	-
H5A25_RS18475	4206419..4207177	-	759	WP_119870824.1	DeoR/GlpR family DNA-binding transcription regulator	-
H5A25_RS18480	4207515..4208429	+	915	WP_119870802.1	NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (39-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17509.30 Da
• Isoelectric Point: 9.0439

>T169611 WP_010281603.1 NZ_CP059962:c4203319-4202837 [Pectobacterium brasiliense]
MGITAPEPLSSSHNLAEFCSQEPSLNEWLKKKALKNHSAGISRVYVICAEGTHQVIGYYCLSTGSVQRNTAPGAYRRNAP
ESLPVIVLGRLAIDQAYSGKGLGVALLKDAIFRTENIALQVGVRALVVHALDENVRNFYLKFAFDPSPVQPLMLLYPIKI

Nucleotide

• Length: 483 bp

>T169611 NZ_CP076645:c611527-611063 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10667.12 Da
• Isoelectric Point: 4.3611

>AT169611 WP_039317395.1 NZ_CP059962:c4203588-4203310 [Pectobacterium brasiliense]
MKPETKEAPINIRAKASQRDLIDMAANLVSKSRTDFMLEAACREAQDILLDQRLFILDNEQFDEFLAELDAPITEERQAR
INDLMSRKSPWE

Nucleotide

• Length: 279 bp

>AT169611 NZ_CP076645:c611862-611527 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A433N1V3

Antitoxin

• AlphaFold DB: A0A0M2EWB4