Detailed information of TA system TA169604

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 629896..630932
• Replicon: chromosome
• Accession: NZ_CP059962
• Organism: Pectobacterium brasiliense strain IPO:3710 NAK:380

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: H5A25_RS02955
• Protein ID: WP_119871526.1
• Coordinates: 630357..630932 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: H5A25_RS02950
• Protein ID: WP_119871525.1
• Coordinates: 629896..630357 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A25_RS02930	624957..625490	+	534	WP_010282870.1	RNA pyrophosphohydrolase	-
H5A25_RS02935	625503..627749	+	2247	WP_039283946.1	phosphoenolpyruvate--protein phosphotransferase	-
H5A25_RS02940	627976..628842	+	867	WP_010286783.1	prolipoprotein diacylglyceryl transferase	-
H5A25_RS02945	628879..629673	+	795	WP_039312476.1	thymidylate synthase	-
H5A25_RS02950	629896..630357	+	462	WP_119871525.1	helix-turn-helix domain-containing protein	Antitoxin
H5A25_RS02955	630357..630932	+	576	WP_119871526.1	PIN domain-containing protein	Toxin
H5A25_RS02960	631112..631615	+	504	WP_119871527.1	prepilin peptidase-dependent protein	-
H5A25_RS02965	631609..632235	+	627	WP_119871528.1	prepilin peptidase-dependent protein	-
H5A25_RS02970	632271..632687	+	417	WP_119871529.1	YgdB family protein	-
H5A25_RS02975	632674..633069	+	396	WP_014914413.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21493.91 Da
• Isoelectric Point: 4.7230

>T169604 WP_119871526.1 NZ_CP059962:630357-630932 [Pectobacterium brasiliense]
MNHSPYPVVLDACVIYPSLLRDLLMHLGLTGLYQPKWTVLIQEEWQRNLLLNNADVTMDKLRNIEHLMNKALPDANVTGF
EQLIPALVLPDADDRHVLAAAIRANAEVIVTANLKDFPATSLSEFGVEAMHPDEFIADLFDLNSALVLKAVRTSRTAFRK
PPFDVDEYLDCLLRQGLPMTVKELDAYRALI

Nucleotide

• Length: 576 bp

>T169604 NZ_CP076628:c2112392-2112258 [Xanthomonas arboricola pv. pruni]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCGGTGTTGTCGGTCGGTATGCTGTCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCTGCGCGCAAGTAA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 17277.78 Da
• Isoelectric Point: 6.2253

>AT169604 WP_119871525.1 NZ_CP059962:629896-630357 [Pectobacterium brasiliense]
MKNTPLNSLSLPAPKEMEAAIRGQRQLATYLSTKLETQEISIQDAHDIAHRIELPTSALTLLMSILGELAAGNAVQIVPV
QAELTTQEAANILNVSRPHMVKLLEEGHLPFHKTGRHRRVLFADLMQYKEQRENESMQAMQELTDRSQEFGFY

Nucleotide

• Length: 462 bp

>AT169604 NZ_CP076628:c2112627-2112475 [Xanthomonas arboricola pv. pruni]
ATGAAGCGACTGCTGACACTGATGGTACTGGGCCTGTTTTCGGCCGGCGTGATGACTGGCTGCAACACCATGGCCGGCGC
TGGCAAGGACATGCAGGGTGCAGGTGAGAAGGTCGAAAAGAAGGCCGACAATTGCAGCGACGGTAAGTGCTGA