Detailed information of TA system TA169603

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 163871..164531
• Replicon: chromosome
• Accession: NZ_CP059962
• Organism: Pectobacterium brasiliense strain IPO:3710 NAK:380

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: H5A25_RS00850
• Protein ID: WP_072061666.1
• Coordinates: 164118..164531 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A1D7Z1Z6
• Locus tag: H5A25_RS00845
• Protein ID: WP_005971623.1
• Coordinates: 163871..164137 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A25_RS00825	159834..160838	-	1005	WP_119870587.1	LacI family DNA-binding transcriptional regulator	-
H5A25_RS00830	160893..161501	-	609	WP_010279828.1	HD domain-containing protein	-
H5A25_RS00835	161932..162579	+	648	WP_010279833.1	hemolysin III family protein	-
H5A25_RS00840	162634..163635	-	1002	WP_010279836.1	tRNA-modifying protein YgfZ	-
H5A25_RS00845	163871..164137	+	267	WP_005971623.1	FAD assembly factor SdhE	Antitoxin
H5A25_RS00850	164118..164531	+	414	WP_072061666.1	protein YgfX	Toxin
H5A25_RS00855	164600..165535	+	936	WP_119870588.1	23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF	-
H5A25_RS00860	165601..165924	-	324	WP_119870589.1	hypothetical protein	-
H5A25_RS00865	165953..166471	-	519	WP_119870590.1	flavodoxin FldB	-
H5A25_RS00870	166814..167200	-	387	WP_048256533.1	acyl-CoA thioesterase	-
H5A25_RS00875	167405..168733	-	1329	WP_039282120.1	MHS family MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-132)

Antitoxin

• Flavinator of succinate dehydrogenase (8-79)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 16325.29 Da
• Isoelectric Point: 10.7911

>T169603 WP_072061666.1 NZ_CP059962:164118-164531 [Pectobacterium brasiliense]
VAQWRCDLRVSWRMQLLSLVVHGLLVLLILLAPWPDGYAWLWLCLVTMVMFGFIRSQRNIKSRQGEIVLLSETTLNWRQQ
EWQIVKRPWLLKNGILLSLQAVNGKDKQQLWLASDSMGDDEWRHLRQILLQQKHWAR

Nucleotide

• Length: 414 bp

>T169603 NZ_CP076623:c3938403-3937897 [Morganella morganii]
TTGAACTTAACGGCACCGGAACCCTTGCAGCAGCATCATGATTTTACCGCTTTTCAGTCAGGTGAGGGGTCCCTTAACCA
TTGGCTGAAAACCCGCGCATTGCAAAATCAGCAATCCGGAGCCTCGCGGACATTTGTCGTCTGCCACCATAACCGGATAA
TGGCCTATTATGCATTATCGACAGGTGTTATTACCTGTTCACAGGCGGCGGGGCGCTTCAGACGTAATATGCCCCCGGAA
ATTCCGGTTATTCTGTTGGGGCGTCTTGCTGTTGACGAAAGTGTAAAAGGCAGAGGGATCGGACGGGGTCTGATAAAAGA
TGCCGCACTGCGGGTCTTGCAGGCCGCCGGGATTGTGGGTATCCGCGGTATTGTTGTCCGTGCTTTATCCGATAATGCCA
GACGTTTTTATGAGCATACCGGTTTTATGCCGTCACCGGCTGATCCGATGCTGCTGATGATTACATTACGGGATCTGCAG
CTGGCAACCGGCATCTATTCTGAATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10507.94 Da
• Isoelectric Point: 5.1392

>AT169603 WP_005971623.1 NZ_CP059962:163871-164137 [Pectobacterium brasiliense]
MEIDNKSRIHWACRRGMRELDIAIMPFFEHDYDTLNDTDKRTFVRLLQSDDPDLFNWLMNHGEPEDGELKRMISLIQTRN
KDRGPVAM

Nucleotide

• Length: 267 bp

>AT169603 NZ_CP076623:c3938714-3938406 [Morganella morganii]
ATGACAAAACACACAGATGAGCAGCATCCTGTGTCCGGCGTGAATATCACCCGTTCAACACTGAATTTACGCATCAAACC
GGAAGATAAAATTCTGATTGATCGCGCAGCGCATGCTGCCGGTAAAAACAGAACAGAGTTCGTACTGGAAGCTGCACGGC
GTGCTGCCGAAGAAACACTGGCAGATTTACGGGTTATTGATGTCAGTACCGATGTCTATCAGCAGTTTATTACCAGACTG
GATATGGCACCACAGTCCGGTGAAGCGCTGAAGAAGACCATGCTGACGAAGGCGCCGTGGGAGAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1D7Z1Z6