Detailed information of TA system TA169596

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1599485..1600269
• Replicon: chromosome
• Accession: NZ_CP059961
• Organism: Pectobacterium brasiliense strain IPO:4057 NAK:249

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: H5A39_RS07215
• Protein ID: WP_119871144.1
• Coordinates: 1599775..1600269 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A3S0Y1Y3
• Locus tag: H5A39_RS07210
• Protein ID: WP_038921611.1
• Coordinates: 1599485..1599778 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A39_RS07185	1594792..1595577	+	786	WP_185893700.1	phosphonate metabolism protein PhnP	-
H5A39_RS07190	1595805..1597559	+	1755	WP_119871142.1	AIPR family protein	-
H5A39_RS07195	1598212..1598460	-	249	WP_119871143.1	helix-turn-helix domain-containing protein	-
H5A39_RS07200	1598545..1598799	-	255	WP_119871224.1	helix-turn-helix domain-containing protein	-
H5A39_RS07205	1598903..1599145	+	243	WP_072010299.1	helix-turn-helix domain-containing protein	-
H5A39_RS07210	1599485..1599778	+	294	WP_038921611.1	DUF1778 domain-containing protein	Antitoxin
H5A39_RS07215	1599775..1600269	+	495	WP_119871144.1	GNAT family N-acetyltransferase	Toxin
H5A39_RS07220	1600695..1602416	+	1722	WP_119871145.1	AAA family ATPase	-
H5A39_RS07225	1602502..1603344	+	843	WP_119871146.1	DNA adenine methylase	-
H5A39_RS07230	1603544..1603849	-	306	WP_119871147.1	type II toxin-antitoxin system toxin CcdB	-
H5A39_RS07235	1603852..1604070	-	219	WP_015855076.1	type II toxin-antitoxin system antitoxin CcdA	-
H5A39_RS07240	1604129..1604872	-	744	WP_119871148.1	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1599229..1608045	8816

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17689.53 Da
• Isoelectric Point: 7.2150

>T169596 WP_119871144.1 NZ_CP059961:1599775-1600269 [Pectobacterium brasiliense]
MISAPEPLRAEHILSSFCCGVESMDNWLKQRAMKNQVTGASRTFVSCDDSKVLAYYSLASSAVATNAAPGRFRRNMPDPI
PVVVLGRLAVDTSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEAREFYLRVGFEPSPIDPMMLMVTLGDLVG
SLSI

Nucleotide

• Length: 495 bp

>T169596 NZ_CP076619:c4743304-4743170 [Xanthomonas arboricola pv. corylina]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCGGTGTTGTCGGTCGGTATGCTGTCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCTGCGCGCAAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10856.44 Da
• Isoelectric Point: 10.1968

>AT169596 WP_038921611.1 NZ_CP059961:1599485-1599778 [Pectobacterium brasiliense]
MPATNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT169596 NZ_CP076619:c4743539-4743387 [Xanthomonas arboricola pv. corylina]
ATGAAGCGACTGCTGACACTGATGGTACTGGGCCTGTTTTCGGCCGGCGTGATGACTGGCTGCAACACCATGGCCGGCGC
TGGCAAGGACATGCAGGGTGCAGGTGAGAAGGTCGAAAAGAAGGCCGACAATTGCAGCGACGGTAAGTGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3S0Y1Y3